Biotinylated human tissue factor (Coagulation Factor III) is a recombinant, site-specifically biotinylated protein produced in HEK293 cells and supplied lyophilized. It is designed for use in binding assays (ELISA, SPR), bioactivity assays, and protein interaction studies requiring oriented immobilization.
- Supplied as 200 μg lyophilized material for flexible dosing.
- Site-specific biotinylation using Avitag enzymatic labeling for consistent orientation.
- Contains a His-tag to facilitate purification and detection.
- Expressed in HEK293 cells to preserve human-like glycosylation.
- Purity >90% as determined by SDS-PAGE and SEC-MALS.
- Low endotoxin (<1.0 EU/μg) appropriate for biochemical assays.
- Demonstrated bioactivity in coagulation and binding assays.
- Stable for 12 months lyophilized at -20°C to -70°C; limited stability after reconstitution.