Reverse Transcription Products
New England Biolabs, Inc. Luna® Universal One-Step RT-qPCR Kit – 200 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
New England Biolabs, Inc. M-MuLV Reverse Transcriptase – 10000 units
Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3 5 exonuclease activity.
New England Biolabs, Inc. LunaScript Multiplex One-Step RT-PCR Kit - 250 reactions
The LunaScript Multiplex One-Step RT-PCR Kit offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. It contains a reaction mix and an enzyme mix featuring Luna WarmStart Reverse Transcriptase and Q5 Hot Start High Fidelity DNA polymerase. The kit is optimized for multiplex target amplification to enable diagnostics pathogen detection and viral genome sequencing.
New England Biolabs, Inc. OneTaq One-Step RT-PCR Kit – 30 reactions
The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase. ProtoScript II Reverse Transcriptase is a mutant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. OneTaq Hot Start DNA Polymerase is mixture of a Hot Start Taq DNA Polymerase combined with a proof-reading DNA polymerase, resulting in high-yield amplification with minimal optimization. The OneTaq One-Step RT-PCR Kit is capable of amplifying long transcripts up to 9 kb in length.
New England Biolabs, Inc. M-MuLV Reverse Transcriptase – 50000 units
Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3 5 exonuclease activity.
New England Biolabs, Inc. LunaScript RT SuperMix - 2500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 g and as low as single copies of RNA.
New England Biolabs, Inc. LunaScript Multiplex One-Step RT-PCR Kit - 50 reactions
The LunaScript Multiplex One-Step RT-PCR Kit offers a streamlined protocol for cDNA synthesis and PCR amplification in a single reaction. It contains a reaction mix and an enzyme mix featuring Luna WarmStart Reverse Transcriptase and Q5 Hot Start High Fidelity DNA polymerase. The kit is optimized for multiplex target amplification to enable diagnostics pathogen detection and viral genome sequencing.
New England Biolabs, Inc. WarmStart RTx Reverse Transcriptase – 250 reactions
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
New England Biolabs, Inc. ProtoScript II Reverse Transcriptase – 10000 units
ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
New England Biolabs, Inc. Luna® Universal Probe One-Step RT-qPCR Kit – 200 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors