Recombinant streptavidin is supplied in a 5 mg format for high affinity binding to biotinylated molecules. It is a versatile capture reagent for biochemical assays, surface immobilization bead based workflows, plate coating, and purification of biotin labeled targets. Applications include Biotin-streptavidin capture, ELISA, pull-down, SPR, BLI, AlphaScreen, assay plate coating, immobilization of biotinylated proteins and nucleic acids. Technical specifications are included below.- Quantity - 5 mg- Binds D-biotin with a dissociation constant, Kd, of 1.0 x 10^-14 mol/L- Activity - Greater than 16 U/mg, 1 unit binds 1 ug of d-biotin- Source - E. coli- Molecular Weight: 13.4 kDa per monomer, single, non-glycosylated polypeptide chain of 128 amino acids, approximately 53.6 kDa per functional tetramer.- Purity - Greater than 90%- Activity - High-affinity biotin binding- Storage - -20 CThis product is for research use only.