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Agglutinins are biological substances that cause specific cells, or other small particles, to clump or stick together and precipitate out of a solution.
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The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
The SeqPlex-i WGA kit allows amplification of small quantities of DNA or from degraded/highly fragmented DNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2.Step 1 In the Pre-amplification/Library Synthesis step (using the Library Preparation Reagents) the template DNA is replicated using primers comprised of semi-degenerate 3- ends with universal 5- ends. As polymerization proceeds displaced single strands serve as new templates for additional primer annealing and extension producing random overlapping replicons flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typically range from 200
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
GenomePlex WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
A caspase-8 substrate; cleavage of the IETD peptide sequence releases p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity
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A deoxyhexose monosaccharide found on N- and O-linked glycans and glycolipids that exists either as a terminal modification of glycan structures or serves as a point of attachment for adding other sugars; plays a role in A and B blood group antigen substructure determination, selectin-mediated leukocyte-endothelial adhesion, and host-microbe interactions
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A para-nitro aniline chromophore cleaved by caspases (Km = 18, 11, 32, 180, and 12 µM for caspases-1, -3, -4, -6, and -7, respectively); not cleaved by caspase-2; monitored colorimetrically at 405 nm
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A deoxyhexose monosaccharide found on N- and O-linked glycans and glycolipids that exists either as a terminal modification of glycan structures or serves as a point of attachment for adding other sugars; plays a role in A and B blood group antigen substructure determination, selectin-mediated leukocyte-endothelial adhesion, and host-microbe interactions
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
A protein fucosylation inhibitor; inhibits fucosylation of IgG1 monoclonal antibodies produced by CHO-DG44 cells at 50 µM; reduces intracellular GDP-fucose levels in CHO-DG44 cells; decreases intracellular fucose levels in A. thaliana seedlings at 100 µM; decreases root length in A. thaliana seedlings
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
A deoxyhexose monosaccharide found on N- and O-linked glycans and glycolipids that exists either as a terminal modification of glycan structures or serves as a point of attachment for adding other sugars; plays a role in A and B blood group antigen substructure determination, selectin-mediated leukocyte-endothelial adhesion, and host-microbe interactions
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
A protein fucosylation inhibitor; inhibits fucosylation of IgG1 monoclonal antibodies produced by CHO-DG44 cells at 50 µM; reduces intracellular GDP-fucose levels in CHO-DG44 cells; decreases intracellular fucose levels in A. thaliana seedlings at 100 µM; decreases root length in A. thaliana seedlings
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
A protein fucosylation inhibitor; inhibits fucosylation of IgG1 monoclonal antibodies produced by CHO-DG44 cells at 50 µM; reduces intracellular GDP-fucose levels in CHO-DG44 cells; decreases intracellular fucose levels in A. thaliana seedlings at 100 µM; decreases root length in A. thaliana seedlings
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More