Acylation Reagents

Chemical compounds designed to aid in mass spectrometric compound identification via acylation.

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Raybiotech Inc RAYBIOTECH INC S-Acylation Detection Kit, Biotin Switch, 40 Sample Kit

The S-Acylation Detection Kit employs a modified biotin-switch method to enable direct visualization of S-acylation, including S-palmitoylation, of proteins via western blot analysis. This kit is capable of biotin-labeling 40 samples, each containing 100-200 μg of total protein.

Utilizes modified biotin-switch method for S-acylation visualization.
Detects S-palmitoylation of proteins via western blot analysis.
Biotin-labels 40 samples, each with 100 to 200 μg of protein.
Employs acyl-biotinyl exchange (ABE) method.
Blocks unmodified free cysteines, then selectively labels acylated cysteines.
Biotinylation detected by western blot or immunoassay.
Avidin resin enriches biotin-labeled acylated proteins/peptides.
Facilitates S-acylation/palmitoylation site mapping via mass spectrometry.

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Regis Technologies Inc GC Regeant, Regis, TFAA (10 x 1 GM)

TFAA: Trifluoroacetic Anhydride is an Acylation Reagents. TFAA is most commonly used for ECD and reacts with alcohols, amines, and phenols. Bases such as triethylamine and trimethylamine can be added to promote reactivity to TFAA. TFAA is frequently used for the confirmation of drugs of abuse and is the most reactive and volatile of the anhydrides.

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Regis Technologies Inc GC Regeant, Regis, HFBA (10 x 1 GM)

HFBA: Heptafluorobutyric Anhydride is an Acylation Reagent. HFBA is frequently used for the confirmation of drugs of abuse and reacts with alcohols, amines, and phenols. Bases such as triethylamine and trimethylamine can be added to promote reactivity to HFBA. HFBA is most commonly used for ECD because its derivatives are most sensitive to ECD.