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Isotype Controls and Standards

Antibodies that cannot bind to the antigen of interest, but match the other properties of the primary antibody in use; can be used as a standard, a blocking agent, to coat proteins, and to estimate non-specific antibody binding sites.
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1

RB705 Mouse IgG1 κ Isotype Control, BD Horizon™

Isotype Control

Regulatory Status RUO
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Purification Method Affinity Chromatography
Gene Alias IgG1, kappa Isotype Control (Anti-KLH)
Conjugate RB705
Applications Flow Cytometry,Control
Form Liquid
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen Keyhole limpet hemocyanin (KLH)
Isotype IgG1 κ
Concentration 0.2 mg/mL
Clone X40
2

RB780 Rabbit IgG Isotype Control, BD Horizon™

Rabbit Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rabbit
Conjugate RB780
Applications Flow Cytometry (Intracellular Staining),Control
Form Liquid
Isotype IgG
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias Rabbit IgG Isotype Control; immunoglobulin gamma1 constant region
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen KLH (Keyhole Limpet Hemocyanin)
Clone EPR25A
3

BV421 Rabbit IgG Isotype Control, BD Horizon™

Rabbit Recombinant Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rabbit
Conjugate Brilliant Violet 421
Applications Flow Cytometry (Intracellular Staining),Control
Form Liquid
Isotype IgG
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias Rabbit IgG Isotype Control; immunoglobulin gamma1 constant region
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Recombinant Monoclonal
Immunogen KLH (Keyhole Limpet Hemocyanin)
Clone EPR25A
4

BV480 Rabbit IgG Isotype Control, BD Horizon™

Rabbit Recombinant Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rabbit
Conjugate Brilliant Violet 480
Applications Flow Cytometry (Intracellular Staining),Control
Form Liquid
Isotype IgG
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias Rabbit IgG Isotype Control; immunoglobulin gamma1 constant region
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Recombinant Monoclonal
Immunogen KLH (Keyhole Limpet Hemocyanin)
Clone EPR25A
5

anti-IgG2a κ, Rat, APC, Clone: R35-95, Isotype Control, BD

For research applications

Content And Storage Undiluted at 4°C
Purification Method Affinity Purified
Host Species Rat
Conjugate APC
Applications Flow Cytometry (Intracellular Staining)
Immunogen Mouse Pooled Immunoglobulin
Isotype IgG2a κ
Clone R35-95
6

RB780 Rat IgG1 κ Isotype Control, BD Horizon™

Isotype Control

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rat
Conjugate RB780
Applications Flow Cytometry,Control
Form Liquid
Isotype IgG1 κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Chromatography
Gene Alias IgG1 kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Mouse immunoglobulin
Clone R3-34
7

IgG1, κ Mouse, R718, Isotype Control, BD Horizon™

Mouse Monoclonal Antibody

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Target Species Not Applicable
Host Species Mouse
Conjugate R718
Applications Flow Cytometry,Flow Cytometry,Flow Cytometry
Form Liquid
Isotype IgG1 κ
Concentration 0.2 mg/mL
Antigen IgG1, κ
Regulatory Status RUO
Gene Alias IgG1, kappa; Ighg1, Igh-4, γg1; Igk-C, IGKC, Ig κ
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Clone MOPC-21
8

RB705 Mouse IgG2a κ Isotype Control, BD Horizon™

Isotype Control

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Mouse
Conjugate RB705
Applications Flow Cytometry,Control
Form Liquid
Isotype IgG2a κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Chromatography
Gene Alias IgG2a kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen TNP-keyhole limpet hemocyanin
Clone G155-178
9

IgG1, κ Mouse, RB545, Isotype Control, BD Horizon™

Mouse Monoclonal Antibody

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Host Species Mouse
Conjugate RB545
Applications Flow Cytometry
Form Purified
Isotype IgG1 κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Purified
Gene Alias IgG1, kappa Isotype Control (Anti-KLH)
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Keyhole limpet hemocyanin (KLH)
Clone X40
10

IgG2a, κ Mouse, RB545, Isotype Control, BD Horizon™

Mouse Monoclonal Antibody

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Target Species Not Applicable
Host Species Mouse
Conjugate RB545
Applications Flow Cytometry
Form Purified
Isotype IgG2a κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Purified
Gene Alias IgG2a kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen TNP-keyhole limpet hemocyanin
Clone G155-178
11

RY655 Mouse IgG2a, kappa; Isotype Control, BD Horizon™

Isotype Control

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Mouse
Conjugate RY655
Applications Flow Cytometry,Control,Flow Cytometry (Intracellular Staining)
Form Purified
Isotype IgG2a κ
Concentration 0.2 mg/mL
Antigen IgG2a
Regulatory Status RUO
Purification Method Affinity chromatography
Gene Alias IgG2a kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen TNP-keyhole limpet hemocyanin
Primary or Secondary Isotype Control (Primary)
Clone G155-178
12

RY775 Rat IgG1, κ Isotype Control, BD Horizon™

Rat Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rat
Conjugate RY775
Applications Flow Cytometry,Control,Flow Cytometry (Intracellular Staining)
Form Liquid
Isotype IgG1 κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias IgG1 kappa Isotype Control
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Mouse immunoglobulin
Clone R3-34
13

RB613 Rat IgG1, κ Isotype Control, BD Horizon™

Rat Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Rat
Conjugate RB613
Applications Flow Cytometry,Control,Flow Cytometry (Intracellular Staining)
Form Liquid
Isotype IgG1 κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias IgG1 kappa Isotype Control
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Mouse immunoglobulin
Clone R3-34
14

RB824 Mouse IgG2a, κ Isotype Control, BD Horizon™

Mouse Monoclonal Antibody

Content And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Target Species Not Applicable
Host Species Mouse
Conjugate RB824
Applications Flow Cytometry,Control,Flow Cytometry (Intracellular Staining)
Form Liquid
Isotype IgG2a κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Gene Alias IgG2a kappa Isotype Control
Product Type Antibody
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen TNP-keyhole limpet hemocyanin
Clone G155-178
15

Mouse IgG2A Isotype Control, R&D Systems™

Mouse Monoclonal Antibody has been used in 7 publications

Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Target Species Mouse
Host Species Mouse
Conjugate Unconjugated
Applications Control
Isotype IgG2a
Concentration LYOPH
Antigen IgG2a
Regulatory Status RUO
Purification Method Protein A or G purified from hybridoma culture supernatant
Dilution Control
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. with No Preservative
Immunogen KLH
Classification Monoclonal
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Test Specificity Recognizes KLH (Keyhole Limpet Hemocyanin). In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.
Clone 133304