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Isotype Controls and Standards

Antibodies that cannot bind to the antigen of interest, but match the other properties of the primary antibody in use; can be used as a standard, a blocking agent, to coat proteins, and to estimate non-specific antibody binding sites.
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2

Alexa Fluor™ 488 Mouse IgG2b, κ Isotype Control, BD Pharmingen™

Mouse Isotype Control

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Host Species Mouse
Conjugate Alexa Fluor 488
Applications Flow Cytometry
Form Liquid
Isotype IgG2b κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Chromatography
Gene Alias Mouse IgG2b kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen MPC-11 Mouse Myeloma Cell Line
Clone MPC-11
3

IgG Rabbit, PE, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate R-PE
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
4

BV421 Mouse IgG2b, κ Isotype Control, BD Horizon™

Mouse Isotype Control

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Host Species Mouse
Conjugate Brilliant Violet 421
Applications Flow Cytometry,Control
Form Liquid
Isotype IgG2b κ
Concentration 0.2 mg/mL
Regulatory Status RUO
Purification Method Affinity Chromatography
Gene Alias Mouse IgG2b kappa Isotype Control
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Immunogen MPC-11 Mouse Myeloma Cell Line
Clone MPC-11
5

IgG Rabbit, Alexa Fluor 488, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 488
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
6

IgG Rabbit, APC, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate APC
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG control for flow cytometry applications.
Clone 60024B
7

IgG Rabbit, Fluorescein, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Fluorescein
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
8

IgG Rabbit, Alexa Fluor 647, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 647
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG control for flow cytometry applications.
Clone 60024B
9

IgG Rabbit, PerCP, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate PerCP
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 10 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
10

IgG Rabbit, Alexa Fluor 405, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 405
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
11

IgG Rabbit, Alexa Fluor 700, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 700
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
12

IgG Rabbit, Alexa Fluor 594, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 594
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
13

IgG Rabbit, Alexa Fluor 750, Clone: 60024B, Isotype Control, R&D Systems™

Rabbit Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Rabbit
Host Species Rabbit
Conjugate Alexa Fluor 750
Applications Control
Form Purified
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Protein A or G purified from cell culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as rabbit IgG isotype control for flow cytometry applications.
Clone 60024B
14

Mouse IgG1, Alexa Fluor 647, Isotype Control, R&D Systems™

Mouse Monoclonal Antibody

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Mouse
Host Species Mouse
Conjugate Alexa Fluor 647
Applications Control
Form Supernatant
Isotype IgG1 κ
Antigen IgG1
Regulatory Status RUO
Purification Method Protein A or G purified from hybridoma culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias IgG1, Igh-4, RGD1359539, VH7183
Formulation Supplied in a saline solution containing BSA and Sodium Azide.
Immunogen KLH
Classification Monoclonal
Primary or Secondary Secondary
Test Specificity Selected for use as mouse IgG 1 isotype control for flow cytometry applications.
Clone 11711
15

Mouse IgG1 Alexa Fluor™ 488-conjugated Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 6 publications

Content And Storage Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Target Species Mouse
Host Species Mouse
Conjugate Alexa Fluor 488
Applications Control
Isotype IgG1 κ
Antigen IgG1
Gene Symbols IGHG1
Regulatory Status RUO
Purification Method Protein A or G purified from hybridoma culture supernatant
Dilution Control 5 uL/10^6 cells
Gene Alias IgG1, Igh-4, RGD1359539, VH7183
Formulation Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
Immunogen KLH
Classification Monoclonal
Test Specificity Selected for use as mouse IgG 1 isotype control for flow cytometry applications.
Clone 11711