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Isotype Controls and Standards

Antibodies that cannot bind to the antigen of interest, but match the other properties of the primary antibody in use; can be used as a standard, a blocking agent, to coat proteins, and to estimate non-specific antibody binding sites.
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1

IgG1, κ Mouse, Alexa Fluor™ 700, Clone: X40, Isotype Control, BD Pharmingen™

Mouse Monoclonal Antibody

Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Target Species Not Applicable
Host Species Mouse
Conjugate Alexa Fluor 700
Applications Flow Cytometry
Form Purified
Isotype IgG1 κ
Concentration 0.2 mg/mL
Antigen IgG1, κ
Regulatory Status RUO
Purification Method Affinity Chromatography
Gene Alias IgG1, kappa Isotype Control (Anti-KLH)
Formulation Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Classification Monoclonal
Immunogen Keyhole limpet hemocyanin (KLH)
Primary or Secondary Primary
Clone X40
2

BV421 Mouse IgG1, κ Isotype Control, BD Horizon™

Mouse Isotype Control

Regulatory Status RUO
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Purification Method Affinity Chromatography
Gene Alias IgG1, kappa; Ighg1, Igh-4, γg1; Igk-C, IGKC, Ig κ
Host Species Mouse
Conjugate Brilliant Violet 421
Applications Flow Cytometry,Control
Form Liquid
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Classification Monoclonal
Isotype IgG1 κ
Concentration 0.2 mg/mL
Clone MOPC-21 (also known as MOPC21; MOPC 21)
3

Invitrogen™ Rabbit IgG Recombinant Isotype Control (RbNP15), Alexa Fluor™ 488, eBioscience™

Rabbit Recombinant Monoclonal Isotype Control

EDGE ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Target Species Not Applicable
Host Species Rabbit
Conjugate Alexa Fluor 488
Applications Control,Flow Cytometry
Form Liquid
Isotype IgG
Concentration 0.5 mg/mL
Antigen Rabbit IgG
Regulatory Status RUO
Purification Method Protein A
Gene Alias Immunoglobulin G; ImmunoglobulinG; Not Applicable
Product Type Isotype Control
Formulation PBS, proprietary buffer with 0.09% sodium azide; pH 7.2-7.4
Classification Recombinant Monoclonal
Primary or Secondary Isotype Control (Primary)
Clone RbNP15
4

Invitrogen™ Rabbit IgG Recombinant Isotype Control (RbNP15), Alexa Fluor™ 647, eBioscience™

Rabbit Recombinant Monoclonal Isotype Control

EDGE ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Target Species Not Applicable
Host Species Rabbit
Conjugate Alexa Fluor 647
Applications Control,Flow Cytometry
Form Liquid
Isotype IgG
Concentration 0.2 mg/mL
Antigen Rabbit IgG
Regulatory Status RUO
Purification Method Protein A
Gene Alias Immunoglobulin G; ImmunoglobulinG; Not Applicable
Product Type Isotype Control
Formulation PBS, proprietary buffer with 0.09% sodium azide; pH 7.2-7.4
Classification Recombinant Monoclonal
Primary or Secondary Isotype Control (Primary)
Clone RbNP15
6

MilliporeSigma™ Calbiochem™ Human IgG, Fab Fragment, Plasma

Native IgG Fab fragment derived from normal human plasma IgG

8

IgG1 Kappa Human, Isotype Control, R&D Systems™

A Novus product, part of R&D Systems. Human Polyclonal Isotype Control

Antigen IgG1 Kappa
Regulatory Status RUO
Content And Storage Store at 4°C.
Target Species Human
Host Species Human
Conjugate Unconjugated
Applications Control,ELISA
Product Type Isotype Controls
Formulation Borate buffered saline pH 8.2
Classification Polyclonal
Immunogen Human myeloma serum
Isotype IgG1 κ
Concentration 0.5 mg/ml
10

IgG3 Lambda Human, Isotype Control, R&D Systems™

A Novus product, part of R&D Systems. Human Polyclonal Isotype Control

Antigen IgG3 Lambda
Regulatory Status RUO
Content And Storage Store at 4°C.
Target Species Human
Host Species Human
Conjugate Unconjugated
Applications Control,ELISA
Product Type Isotype Controls
Formulation Borate buffered saline pH 8.2
Classification Polyclonal
Immunogen Human myeloma serum
Isotype IgG3 λ
Concentration 0.5 mg/ml
11

IgA Lambda Human, Isotype Control, R&D Systems™

A Novus product, part of R&D Systems. Human Polyclonal Isotype Control

Antigen IgA λ
Regulatory Status RUO
Content And Storage Store at 4°C.
Target Species Human
Host Species Human
Conjugate Unconjugated
Applications Control,ELISA
Product Type Isotype Controls
Formulation Borate buffered saline pH 8.2
Classification Polyclonal
Immunogen Human myeloma serum
Isotype IgA λ
Concentration 0.5 mg/ml
12

MilliporeSigma™ Human IgE, Myeloma, Isotype Control

Human Isotype Control Antibody

Antigen IgE, Myeloma
Regulatory Status RUO
Content And Storage −20°C
Host Species Human
Form Liquid
Formulation In 150mM NaCl, 15mM potassium phosphate buffer, pH 7.4.
Isotype IgE
Primary or Secondary Isotype Control (Primary)
Test Specificity Native IgE from human myeloma plasma.
14

IgM Lambda Human, Isotype Control, R&D Systems™

A Novus product, part of R&D Systems. Human Polyclonal Isotype Control

Content And Storage Store at 4°C.
Target Species Human
Host Species Human
Conjugate Unconjugated
Applications Control,ELISA
Isotype IgM λ
Concentration 0.5 mg/ml
Antigen IgM Lambda
Regulatory Status RUO
Product Type Isotype Controls
Gene ID (Entrez) 3507
Formulation Borate buffered saline pH 8.2
Classification Polyclonal
Immunogen Human myeloma serum
15

MilliporeSigma™ Polyclonal Mouse IgG

Mouse IgG Polyclonal Antibody control validated for use in ELISA.