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The PathScan™ Mono-Methyl-Histone H3 (Lys4) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) designed to detect endogenous levels of histone H3 when mono-methylated at Lys4. This kit utilizes a total Histone H3 rabbit monoclonal antibody coated onto microwells to capture histone H3 from cell lysates, followed by a biotinylated detection antibody and HRP-linked streptavidin for colorimetric quantification proportional to the amount of mono-methylated protein present.
Solid phase sandwich ELISA format
Detects endogenous levels of histone H3 mono-methylated at Lys4
Utilizes biotinylated detection antibody and HRP-linked streptavidin
Supplied with a 96-well strip plate in a support frame for custom configurations
Includes cell lysis buffer and all necessary reagents for detection
Validated for use with human samples
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