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Biofluids containing aggregate cellular material produced by the disintegration or dissolution of a variety of cell lines; designed for use in immunoprecipitation and immunoassay procedures.
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The HIF-1alpha Whole Cell Lysate Kit from Mesoscale Discovery is used to detect HIF-1alpha protein in cell lysates The kit contains a 96-well plate coated with capture antibody detection antibody sample diluent wash buffer readout buffer and a vial of lyophilized recombinant HIF-1alpha standard The assay is performed by adding sample or standard detection antibody washing adding readout buffer and analyzing on an electrochemiluminescence plate reader The kit can quantitatively measure HIF-1alpha levels from cell lysates with high sensitivity and wide dynamic range
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