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Laminin is a glycoprotein with a molecular weight of approximately 900 kDa found in the basement membrane of most tissues It is composed of three chains a1 1 and 1 It binds to collagen type IV and heparan sulfate playing a crucial role in the structure and function of the basement membrane In cell culture it primarily mediates adhesion between epithelial cells endothelial cells and hepatocytes and also promotes axonal outgrowth in neurons as well as differentiation and chemotaxis It is closely associated with cancer metastasis and highly metastatic cancer cells are known to express a high number of laminin receptors on their cell surfaces
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The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix about 400 nm in length composed of at least two different chain subunits termed1 (IV) and2 (IV) which may be composed of the same or different molecules. Upon proteolysis fragments of 125 kDa 95 kDa and 60 kDa are released from a parent1 (IV) chain whereas fragments of 115 kDa 80 kDa and 45 kDa apparently originate from a distinct2 (IV) chain.
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