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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 15 of 221 results
1

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
GSA_VA

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
2

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
3

Cytiva HisTrap™ FF Columns: 5 x 5mL
GSA_VA

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

EDGE
4

Cytiva HisTrap™ HP Prepacked Columns
GSA_VA

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
5

Cytiva HisTrap™ FF Columns: 16 x 25mm
GSA_VA

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
6
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Cytiva HisTrap™ excel Prepacked Columns
GSA_VA

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
7

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
8

Cytiva His Mag Sepharose™ excel Chromatography Media
GSA_VA

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Particle Size 63 μm
Type Chromatography Media
Content And Storage 4°C to 30°C
pH Range 2 to 12
For Use With (Application) Designed to simplify small-scale purification and screening of His-tagged Proteins secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
Support Type Highly cross-linked spherical Agarose including Magnetite
9

Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.62 in.
Bed Volume 5 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 4 to 13
Diameter (Metric) Inner 16 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
Particle Size 45 to 165 μm
Ligand Type Glutathione
Flow Rate <20 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
10

Cytiva™ Protein Select™ Resin

An affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag.

11

Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

Ligand Type Glutathione
Flow Rate <4 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 4 to 13
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Support Type 4% Highly Cross-linked Agarose
12

Cytiva SOURCE™ 15Q

Strong anion exchanger with high chemical stability for wide range of working conditions with good resistance to cleaning conditions at high pH

Particle Size 15μm
Buffer 20mM N-methylpiperazine, 20mM Piperazine, 20mM Bis-Tris, 20mM Triethanolamine, 20mM Tris, 20mM Diethanolamine, 20mM Ethanolamine, Glycine, 20mM 1,3-diamino-propane, 20mM Piperidine
Ligand Type Quaternary Ammonium
Flow Rate 1800 cm/hr.
Height (Metric) 100mm
Content And Storage 4° to 30°C
Matrix 15Q Chromatography Media
pH Range 2 to 12
Product Type Source 15Q chromato graphy media
For Use With (Application) Polishing steps in both research and industrial applications
Support Type Polystyrene/Divinylbenzene
Max. Pressure 5 bar
Height (English) 3.93 in.
13

Cytiva Tricorn Glass Tubes

Glass tubes coated with plastic film protects individuals in the laboratory from harm if the column is dropped or operated outside its specification.

Product Type Glass tube
Column Type Column Accesory
Diameter (Metric) Inner 10 mm
Material Borosilicate glass
14

Cytiva Glutathione Sepharose™ 4 Fast Flow Media
GSA_VA

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Particle Size 90 μm
Ligand Type Glutathione and 10-carbon linker arm
Flow Rate <450 cm/hr.
Type Chromatography Media
Content And Storage 4°C to 30°C, 20% Ethanol
Matrix Highly cross-linked 4% agarose
Kit Contents 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Description Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Max. Operating Pressure base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
pH Range 3 to 12
Binding Capacity >10 mg/mL
Support Type 4% Highly Cross-linked Agarose
15

Cytiva GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Particle Size 34 μm
Ligand Type Glutathione
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Description GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
For Use With (Application) Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Support Type 4% Highly Cross-linked Agarose