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Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.
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1

Cytiva HiTrap™ Heparin HP Columns
GSA_VA

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
2

Cytiva Sephadex™ Ion Exchange Media

Separate molecules over broad molecular weight and pH ranges

Height (Metric) 5 to 15cm
Content And Storage 4° to 30°C
Format Solid Spherical Bead
Product Type Sephadex Ion Exchange Media
For Use With (Equipment) Column or Batch Techniques
For Use With (Application) Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques
Support Type Cross-linked Dextran
Height (English) 2 to 5.9 in.
3

Cytiva Superose™ Media Prep Grade Lab Packs

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Particle Size 30 to 40 μm
Flow Rate 40 cm/hr.
Bed Volume ≈100 mL
pH Range 3 to 12
For Use With (Equipment) AKTA™ design pump or system
Sample Type Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules
Quantity Each
For Use With (Application) Separations of Proteins and other biomolecules according to size
Support Type Highly Cross-linked Agarose
4

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Content And Storage 4° to 30°C
Bed Volume 15 to 20mL/g in Distilled Water
Format Solid Bead
pH Range 2 to 10
Product Type Sephadex G-100 Media
Sample Type Large biomolecules
Quantity 100 g
For Use With (Application) Useful for desalting and Buffer exchange of very large biomolecules
Support Type Cross-linked Dextran
Max. Pressure 0.096 bar
5
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Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel

Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration

6

Cytiva Superdex™ 30 Prep Grade

Offers high resolution with short run times and good recovery

7

Cytiva Superdex 30 PG size exclusion chromatography media prepacked into HiLoad 26/600 column
GSA_VA

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.

8

Cytiva Sephadex™ LH-20
GSA_VA

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
9

Cytiva Sephacryl™ S-300 High Resolution Media
GSA_VA

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

10

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
GSA_VA

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Particle Size 25 to 75 μm
Height (Metric) 60cm
pH Range 3 to 11
Product Type Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Quantity Each
Material Polypropylene
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
Height (English) 23.62 in.
11

Cytiva HiPrep™ Sephacryl™ S-400 HR Columns

Maximum recovery and minimum nonspecific adsorption

12

Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

13

Cytiva Superdex™ 200 Prep Grade
GSA_VA

Produce separation medium with high selectivity and resolution. Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D

14

Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Height (Metric) 30cm
Content And Storage 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
Bed Volume 4 to 6mL/g in Distilled Water
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex G-25 Media
Diameter (Metric) Inner 2.6cm
For Use With (Application) Desalting and Buffer exchange and removal free label in industrial applications
Support Type Cross-linked Dextran
Height (English) 11.81 in.
15

Cytiva Sephacryl™ S-500 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Height (Metric) 15cm
Content And Storage 4° to 30°C
Bed Volume 19 to 230L
pH Range 3 to 11
Diameter (Metric) Inner 40 to 140cm
For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
Max. Pressure 100kPa
Particle Size 25 to 75 μm
Flow Rate >125 cm/hr.
Format Solid Spherical Bead
Product Type Sephacryl HR Media
Sample Type Polysaccharides, macromolecules and small plasmids
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Height (English) 5.9 in.