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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (142)
Magnetic Affinity Separation Particles (25)
Spin Columns and Gravity-flow Columns (10)
Gel Filtration Media and Columns (6)
Protease Inhibitors and Phosphatase Inhibitors (4)
Cell Lysis Detergents (2)
Cell Lysis Enzymes (2)
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In vitro Protein Expression Systems (1)
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1 – 15 of 175 results
1

Cytiva Glutathione Sepharose™ High Performance Media

For high resolution purification of Glutathione S transferase (GST) tagged proteins

Particle Size 34 μm
Ligand Type Glutathione
Flow Rate <600 cm/hr.
Type Chromatography Media
Content And Storage 4°C to 30°C, 20% Ethanol
Matrix Highly crossed-linked agarose, 6%
Description Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority.
pH Range 3 to 12
Binding Capacity >Approx. 10 mg GST-tagged Protein/mL Resin
Support Type 6% Highly Cross-linked Agarose
2

Cytiva Ni Sepharose™ High Performance Media
GSA_VA

Precharged with Ni2+ for optimal purification of histidine-tagged proteins. Cytiva Lifescience™ Ni Sepharose™ High Performance Media shows high binding capacity and compatibility.

Particle Size 34μm
Content And Storage 4° to 30°C
Matrix Ni-Sepharose
pH Range 3 to 12
Product Type Ni Sepharose HP chromato graphy media
Support Type 6% Cross-linked Agarose
3

Cytiva Dextrin Sepharose™ High Performance Media
GSA_VA

Provide convenient affinity purification of recombinant proteins with maltose binding protein (MBP) tags

Particle Size 34 μm
Matrix Affinity Resin
Material Dextrin Sepharose
4
Promotions Available

Cytiva Streptavidin Sepharose High Performance Resin

Affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.

5

Cytiva Streptavidin Sepharose™ High Performance Medium
GSA_VA

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

6

Cytiva HiScreen™ Sepharose™ Prepacked High Performance HIC Columns

Designed for method optimization and parameter screening

Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
pH Range 3 to 13
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7 mL
Material Polypropylene
For Use With (Application) Convenient process development optimization parameter screening
Max. Pressure 3 bar
Particle Size 24 to 44μm
Flow Rate <150 cm/hr.
Height (English) 3.93 in.
7

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns

Designed for method optimization and parameter screening

Buffer 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <150 cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
8
Promotions Available

Thermo Scientific™ Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits

Easily isolate and culture highly viable functional primary cardiomyocytes

EDGE
9
Promotions Available

Thermo Scientific™ Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits

Easily isolate and culture highly viable functional primary neurons

EDGE
10
Promotions Available

Thermo Scientific™ Pierce™ Crosslink IP Kit
GSA_VA

Reagent kit and protocol for crosslinking IP antibodies to Protein A/G agarose to enable antigen immunoprecipitation without antibody contamination.

EDGE
11
Promotions Available

Thermo Scientific™ Pierce™ Direct IP Kit

Immobilize IP antibodies (any species or class) on agarose beads without Protein A/G to immunoprecipitate antigens without antibody interference.

EDGE
12

Cytiva Tricorn Columns
GSA_VA

Tricorn columns are compatible with high flow rates and are designed for high-performance media such as Sepharose, Superdex, and SOURCE.

Includes Glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock and filter holder
Product Type Tricorn Columns (Empty)
Quantity 1 Ea.
Material Borosilicate glass
For Use With (Application) High resolution liquid chromatography of biomolecules performed at medium to high pressures
Support Type Polypropylene filter
13

Cytiva Tagged Column Package, His (2-step Purification Protocol)

Combines affinity with size exclusion chromatography to obtain highly pure his-tagged proteins

14

Cytiva SOURCE™ Tricorn™ Columns for IEX

Source 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

Buffer All common ion exchange Buffers
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.18 in.
Bed Volume 1.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 4.6mm
Quantity 1 x 1.7mL
Material PEEK
For Use With (Application) High performance ion exchange chromato graphy of Proteins, Peptides, Polynucleotides and other biomolecules
Max. Pressure 40 bar
Particle Size 15μm
Flow Rate <5 mL/min.
Support Type Polystyrene/Divinylbenzene
Height (English) 3.93 in.
15

Cytiva GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Particle Size 34 μm
Ligand Type Glutathione
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Description GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
For Use With (Application) Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Support Type 4% Highly Cross-linked Agarose