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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (344)
Gel Filtration Media and Columns (89)
Protease Inhibitors and Phosphatase Inhibitors (79)
Spin Columns and Gravity-flow Columns (42)
Affinity Gels (28)
Miscellaneous Immobilization Supports (28)
Magnetic Affinity Separation Particles (18)
Cell Lysis Detergents (6)
Biotin-Binding Affinity Resins and Beads (5)
Miscellaneous Immobilized Affinity Ligands (4)
Cell Lysis Enzymes (2)

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1 – 15 of 459 results
1

Cytiva Life Sciences rProtein A GraviTrap™

Includes ten ready-to-use columns prepacked with 1ml of rProtein A Sepharose Fast Flow

2

Cytiva Life Sciences Protein G GraviTrap™

Includes ten ready-to-use columns prepacked with 1ml of Protein G Sepharose 4 Fast Flow

3

Cytiva Life Sciences rProtein A Protein GraviTrap™

Includes ten ready-to-use columns prepacked with 1ml of a mixture of 50% rProtein A Sepharose Fast Flow and 50% Protein G Sepharose 4 Fast Flow

4

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
GSA_VA

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
5

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
6

Cytiva HisTrap™ FF Columns: 5 x 5mL
GSA_VA

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.

EDGE
7

Cytiva CNBr-Activated Sepharose™ Medium
GSA_VA

Use for coupling antibodies or other large proteins containing -NH2 groups to the Sepharose media. CNBr-Activated Sepharose™ Medium is a pre-activated media perfect for custom affinity applications.

EDGE
8

Cytiva HisTrap™ HP Prepacked Columns
GSA_VA

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
9

Cytiva HisTrap™ FF Columns: 16 x 25mm
GSA_VA

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
10
Promotions Available

Cytiva HisTrap™ excel Prepacked Columns
GSA_VA

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

EDGE
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 2 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
11

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

EDGE
Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
12

Cytiva HiTrap™ Heparin HP Columns
GSA_VA

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

EDGE
Particle Size 34 μm
Ligand Type 10mg Heparin/mL medium
Height (Metric) 2.5cm
Content And Storage 4° to 30°C
pH Range 5 to 10
Product Type Heparin HP Column
For Use With (Equipment) Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material Polypropylene
For Use With (Application) Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins
Support Type 6% Highly Cross-linked Agarose
Max. Pressure 42psi
Height (English) 0.98 in.
13

Cytiva His Mag Sepharose™ excel Chromatography Media
GSA_VA

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Particle Size 63 μm
Type Chromatography Media
Content And Storage 4°C to 30°C
pH Range 2 to 12
For Use With (Application) Designed to simplify small-scale purification and screening of His-tagged Proteins secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma
Support Type Highly cross-linked spherical Agarose including Magnetite
14

Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.62 in.
Bed Volume 5 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 4 to 13
Diameter (Metric) Inner 16 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
Particle Size 45 to 165 μm
Ligand Type Glutathione
Flow Rate <20 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
15

Cytiva™ Protein Select™ Resin

An affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag.