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Specific assay kits designed measure the activation levels of small, active GTPases by isolating them via their specific downstream effectors. Kits employ molecular methods including pull-down affinity purification, electrophoresis, and western blotting.
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This kit detects RAB10 in human serum plasma tissue homogenates and cell lysates offering a broad detection range of 39 pg/mL to 2500 pg/mL with a sensitivity of 9 77 pg/mL Designed for quantitative analysis it employs a sandwich ELISA methodology ensuring high specificity and accuracy The assay requires a short incubation period of 1-5 hours and uses a sample volume of 50-100 L per test Results are measured at 450 nm providing reliable data for research applications such as studying protein expression regulatory mechanisms or disease associations Its optimized protocol balances efficiency and precision making it suitable for laboratories prioritizing robust performance in RAB10 detection across diverse biological matrices
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