Galactosidase Assay Kits

Sigma Aldrich Fine Chemicals Biosciences beta Galactosidase Reporte

-galactosidase (-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of -gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of -gal like O-nitrophenyl--D-galactopyranoside (ONPG). -gal is a cost-effective simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the -galactosidase gene LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Sigma Aldrich Fine Chemicals Biosciences beta-Galactosidase from E.

-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains each constituting 1023 amino acids. It is very specific for D-galactose and requires K or Na and Mg2 to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.