Class-Specific Protein Assay Kits
Biotium Protease Substrates Enzyme Substrates Protease Substrates Chemical Reagents 1/EA
This kit contains recombinant TEV Protease and Control Substrate both expressed from E coli along with 10X Reaction Buffer All kit components are supplied in solution and can be used to cleave affinity tags from fusion proteins using the TEV protease recognition site Unit size 10000 U
Biotium Protease Substrates Enzyme Substrates Protease Substrates Chemical Reagents 1/EA
This kit contains recombinant TEV Protease and Control Substrate both expressed from E coli along with 10X Reaction Buffer All kit components are supplied in solution and can be used to cleave affinity tags from fusion proteins using the TEV protease recognition site Unit size 1000 U
Sigma Aldrich Fine Chemicals Biosciences Human DNA quantitation standard NIST(R) SRM(R) 2372a |
Human DNA quantitation standard NIST(R) SRM(R) 2372a |
Sigma Aldrich Fine Chemicals Biosciences Succinate Dehydrogenase Assay Kit
Succinate Dehydrogenase Assay Kitsufficient for 100 colorimetric tests
Sigma Aldrich Fine Chemicals Biosciences Malic Dehydrogenase from b
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Sigma Aldrich Fine Chemicals Biosciences L-Lactate Dehydrogenase L-
L-lactateNAD oxidoreductase.L-lactate dehydrogenase catalyzes the reversible reduction of pyruvate to L-lactate.
Sigma Aldrich Fine Chemicals Biosciences formate Dehydrogenase from
formateNAD oxidoreductaseformate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Sigma Aldrich Fine Chemicals Biosciences Lactate Dehydrogenase Inhi
A cell-permeable quinolinesulfonamide compound that acts as potent and reversible inhibitor of lactate dehydrogenase A (IC50 2.6 nM for human LDH-A). The inhibition appears to be competitive with respect to NADH and non-competitive with respect to pyruvate. Exhibits high selectivity over LDH-B (IC50 43 nM) and does not affect the activity of 32 other enzymes receptors and ion-channels even at high concentration (~ 10 181M). Rapidly reduces lactate production in hepatocellular (EC50 400 and 588 nM in Snu398 and HepG2 cells respectively) and breast carcinoma cell lines and induces PKM2 activation (EC50 600 nM in Snu398 cells). Shown to increase mitochondrial oxygen consumption rate (EC50 500 and 900 nM in Snu398 and HepG2 cells respectively) and causes a diminution in extracellular acidification rates (EC50 600 nM in both cell lines). Shown to downregulate the ability of A549 cells to form tumorspheres and diminishes the number of CD24/CD44-positive cells in HMLER system and i
Sigma Aldrich Fine Chemicals Biosciences L Lactate Dehydrogenase L
L-lactateNAD oxidoreductase.L-lactate dehydrogenase catalyzes the reversible reduction of pyruvate to L-lactate.
Sigma Aldrich Fine Chemicals Biosciences Dipeptidyl Peptidase IV In5MG
Dipeptidyl Peptidase IV In5MG
Sigma Aldrich Fine Chemicals Biosciences formate Dehydrogenase from
formateNAD oxidoreductaseformate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Sigma Aldrich Fine Chemicals Biosciences LACTATE DEHYDROGENASE ASSAY KI
Lactate Dehydrogenase Assay Kit
Sigma Aldrich Fine Chemicals Biosciences Arginase Activity Assay Kit sufficient for 200 colorimetric tests | 1KT
Arginase Activity Assay Kit sufficient for 200 colorimetric tests | 1KT
Sigma Aldrich Fine Chemicals Biosciences Phospholipid Assay Kit sufficient for 100 colorimetric or fluorometric tests | 1KT
Phospholipid Assay Kit sufficient for 100 colorimetric or fluorometric tests | 1KT
Sigma Aldrich Fine Chemicals Biosciences TEV Protease Biotin tagged
TEV protease is a highly sequence specific serine protease from Tobacco Etch Virus. Due to its high specificity TEV protease is popular for cleavage of recombinant fusion proteins. The optimal sequence for TEV protease cleavage is ENLYfQ\S is however TEV protease is active on a range of substrates with a consensus sequence of EXLYfPhiQ\981 where X is any residue Phi is any large or medium hydrophobic residue and 981 is any small hydrophobic or polar residue (i.e. glycine serine alanine valine cysteine). fusion tag removal in-vitro is the most popular usage of TEV protease. This biotin-tagged TEV protease is expressed in E.coli. Our Biotin-tagged TEV protease does not carry any purification tag other than biotin and is designed to be used for on-column cleavage of fusion proteins containing a TEV protease recognition sequence. This method specifically cleaves the protein of interest from a column-bound fusion protein leaving the purification domain or tag bound to the affinity
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