The Lambda Protein Phosphatase Kit contains reagents engineered to catalyze the removal of phosphate groups from phosphorylated serine, threonine, and tyrosine residues. This Mn2+-dependent protein phosphatase is derived from the bacteriophage lambda ORF221 open reading frame and expressed in E. coli. The kit provides the necessary components for phosphatase reactions, including the enzyme, PMP buffer, and MnCl2 solution.
- Active toward phosphorylated serine, threonine, and tyrosine residues.
- Enzyme concentration of 400,000 units per mL with a specific activity of 800,000 units per mg.
- Unit definition: one unit hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 min at 30°C in a 50 μL reaction volume.
- Includes Lambda Protein Phosphatase, PMP buffer, and MnCl2 for PMP.
- Stable for 24 months when stored at -80°C.