General Cell Based Assays
Enzo Life Sciences PROTEOSTAT Aggresome detection kit (1x100tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT. . Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent . Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Enzo Life Sciences GFP-CERTIFIED FLUOFORTE Calcium assay kit (10x96wells)
Enzo Life Sciences’ GFP-CERTIFIED FLUOFORTE Calcium Assay Kit (high-throughput) provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Relative to other commercially available dyes, the GFP-CERTIFIED FLUOFORTE dye is the brightest and most sensitive fluorescent calcium indicator. The kit provides a homogeneous mix-and-read, no-wash calcium mobilization assay. The homogenous cell-based assay for calcium offers fewer steps, lower variability and an easier protocol for adherent and non-adherent cell lines. In addition, it requires neither a washing step, nor exogenous addition of a quencher dye, which could adversely effect receptor-ligand interaction kinetics. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences Matrix Metalloproteinase-13 (MMP-13) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-13 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-13, a potential therapeutic target. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet. Alternative name: Collagenase 3. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences FLUOR DE LYS-Green SIRT5 fluorometric drug discovery assay kit (100 tests)
The FLUOR DE LYS Green SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats. The Green SIRT5 Fluorescent Activity Assay is based on the unique FLUOR DE LYS-Succinyl GreenSubstrate/Developer combination. The FLUOR DE LYS-Succinyl GreenSubstrate, which comprises a lysine residue, Nε-succinylated on its side-chains, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl Green sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Green sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Enzo Life Sciences Express DNA methylation kit (deep well) (2x96wells)
The Express DNA methylation kit (deep well) features high-throughput (96-well) bisulfite treatment and conversion of DNA for methylation analysis. No preparation is necessary when using the Express Conversion Reagent. Simply add this reagent to a DNA sample and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate.High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing.
Enzo Life Sciences ER-ID Red assay kit (GFP-CERTIFIED) (1x1Kit)
Endoplasmic Reticulum staining with minimal toxicity to cells. Applications: Fluorescence microscopy. Contents: ER-ID Red detection reagent: 50μl. Hoechst 33342 nuclear stain: 50μl. 10X assay buffer: 15ml
Enzo Life Sciences MITO-ID Membrane potential cytotoxicity kit (1x1Kit)
A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity. Applications: HTS, Microplate. Contents: MITO-ID MP Detection Reagent, 200 μL. CCCP Control, 100 μL . 10X Assay Buffer 1: 2.5 mL. 50X Assay Buffer 2: 0.5 mL
Enzo Life Sciences GOLGI ID Green assay kit (1x1Kit)
Dependably localizes to Golgi apparatus with minimal staining of ER. Applications: Fluorescence microscopy. Contents: GOLGI ID Green detection reagent: 50nmoles. Hoechst 33342 nuclear stain: 50μl. 10X assay buffer 1: 15ml. 50X assay buffer 2: 1.2ml
Enzo Life Sciences Express DNA methylation kit (shallow well) (2x96wells)
The Express DNA methylation kit (shallow well) features high-throughput (96-well) bisulfite treatment and conversion of DNA for methylation analysis. No preparation is necessary when using the Express Conversion Reagent. Simply add this reagent to a DNA sample and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing.
Enzo Life Sciences FLUOFORTE Calcium assay kit (96tests)
Fluo-Forte Calcium Assay Kits detect mobilization of intracellular calcium utilizing a fluorogenic calcium-binding dye optimized for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence. Mechanism of Action The fluorogenic calcium-binding dye is provided to the cells as an acetoxymethyl (AM) ester, which is cell-permeable. Once inside the cells, the dye is hydrolyzed by intracellular esterases, which leads to generation of a cell membrane impermeable negatively charged form. In the absence of calcium, the calcium-binding moiety portion of the probe quenches the fluorescence of the fluorophore portion of the probe by photo-induced electron transfer. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS .
Enzo Life Sciences HDAC substrate (fluorogenic) (1mg). CAS: 233691-67-3
A fluorogenic substrate for histone deacetylase (HDAC). Ex(max): 390nm; Em(max): 460nm. Alternative name: Boc-Lys(Ac)-AMC, MAL, Histone deacetylase substrate (fluorogenic), N-(4-Methyl-7-aminocoumarinyl)-Nalpha-(t-butoxycarbonyl)-Nomega-acetyllysineamide. Formula: C23H31N3O6. MW: 445.5. Purity: ≥99%. Appearance: White crystalline solid. Solubility: Soluble in DMSO and 100% ethanol (1mg/ml). Long Term Storage: -20°C. Handling: Protect from light.