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Bioluminescence Assays and Reagents

Luciferase vectors, cell lysis buffers, signal enhancers, transfection reagents, assay kits, and other associated products for optimal luciferase reporter assay performance.
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1

Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)

Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.

EDGE
2

Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.

EDGE
Substrate Vargulin (Cypridina Luciferin)
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
3

Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

EDGE
Substrate Vargulin (Cypridina Luciferin)
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
4

Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

EDGE
Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
5

Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.

EDGE
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Green Renilla Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
6

Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based Renilla luciferase kit.

EDGE
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Green Renilla Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
7

Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit
GSA_VA

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters

EDGE
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Detection Method Bioluminescence
Promoter EF-1α
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Gaussia Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
8

Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.

EDGE
Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
9

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

EDGE
Substrate Coelenterazine, D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Gaussia Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
10

Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.

EDGE
Substrate Coelenterazine
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Gaussia Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
11

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Perform firefly luciferase assays in high-throughput screening applications.

EDGE
Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Cypridina Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Detection Method Bioluminescence
12

Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

EDGE
Substrate Vargulin (Cypridina Luciferin), D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
13

Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

EDGE
Substrate Coelenterazine, D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Green Renilla Luciferase
Type Assay Kit
Format 96-well Plate, 384-well Plate
For Use With (Equipment) Luminometer
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
14

Biotium 5X Firefly Luciferase Lysis Buffer

5X Firefly Lysis Buffer is a concentrated cell lysis buffer that is designed for use with Biotium's Firefly Luciferase Assay Kit 2.0 and Firefly Luciferase Assay Kit (Lyophilized). These kits are supplied with sufficient 5X Firefly Lysis Buffer to perform the stated number of assays with cells grown in 96 to 24 well plates. 5X Firefly Lysis Buffer is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required.

ENCOMPASS_PREFERRED
15

Indigo Biosciences INDIGlo LDR Kit 50 mL of 2x- concentration Luciferase Detection Reagent and Dilution Buffer

INDIGlo Luciferase Detection Reagent from INDIGO Biosciences is a glow type luciferase detection reagent designed for the quantification of firefly and beetle luciferase expression in mammalian cells. The INDIGlo Luciferase Detection Reagent kit is formulated as a 2x-concentrated reagent which contains both the Luciferase Detection Reagent (LDR) and Dilution Buffer and can be used for both homogenous and non-homogenous assay applications. With a signal duration of greater than 2 hours and with a less than 10% fluctuation in signal, the highly sensitive and stable luminescence signal of INDIGlo is ideal for the cost-effective processing of cell culture plates ranging from 96 to 1536-well format.

ENCOMPASS_PREFERRED