Protein Assays
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Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)
Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Thermo Scientific™ Pierce™ Microplate BCA Protein Assay Kit - Reducing Agent Compatible
Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.
Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.
| Substrate | Vargulin (Cypridina Luciferin) |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
| Substrate | Vargulin (Cypridina Luciferin) |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Renilla Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life approx. 3 hour) in the presence of Renilla luciferase reporters.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Gaussia luciferase reporters
| Compatible Cells | Mammalian Cells |
|---|---|
| Substrate Type | Luciferase Substrate |
| Detection Method | Bioluminescence |
| Promoter | EF-1α |
| Substrate | Coelenterazine |
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
Thermo Scientific™ Pierce™ Renilla Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based Renilla luciferase kit.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Green Renilla Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Invitrogen™ Quant-iT™ Protein Assay Kit
For easy and accurate protein quantitation
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit
Assay regulatory element transcriptional activity in mammalian culture media or whole cell lysates with this highly sensitive flash-based Gaussia luciferase kit.
| Substrate | Coelenterazine |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit
Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.
| Substrate | Coelenterazine, D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Gaussia Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit
Perform firefly luciferase assays in high-throughput screening applications.
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase, Cypridina Luciferase |
| Type | Assay Kit |
| Format | 96-well Plate, 384-well Plate |
| For Use With (Equipment) | Luminometer |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Detection Method | Bioluminescence |