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Reagents, kits, and instruments for use during the extraction of proteins from bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, flowering plants, etc. Products include lysis buffers, master mixes, and solubilizing solutions.
Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.
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1% SDS Lysis Buffer is great for fully solubilizing proteins in preparation for Western blotting! It is suitable for both organs and tissue culture cells. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
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Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
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CelLytic Y Cell Lysis Reagent is versatile phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.
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CelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.
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