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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

ENCOMPASS_PREFERRED
Format Kit
Product Type Cloning Kit
Cloning Method TOPO™-TA
Bacterial or Yeast Strain TOP10
Vector TOPO-TA Cloning Vectors
Product Line One Shot
2

Invitrogen™ FluoReporter™ lacZ/Galactosidase Quantitation Kit

Reaction produces blue fluorescent product

EDGE ENCOMPASS_PREFERRED
3

Invitrogen™ ImaGene Green™ C12FDG lacZ Gene Expression Kit

Substrates cleaved within cell for fluorescent product

EDGE ENCOMPASS_PREFERRED
4

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System

Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the

ENCOMPASS_PREFERRED
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Luciferase
Substrate Type Luciferase Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Luciferin
Assay Luciferase Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer
Product Type Luciferase Reporter Gene Assay System
Sample Type Cell Cultures
Product Line Luc-Screen, NovaBright
5

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

ENCOMPASS_PREFERRED
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Gal-Screen, NovaBright
6

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

ENCOMPASS_PREFERRED
7

Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.

ENCOMPASS_PREFERRED
Content And Storage This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Plus™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
8

Invitrogen™ Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates. SensitiveThe chemiluminescent assay exhibits over three o

ENCOMPASS_PREFERRED
Content And Storage This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Beta-Galactosidase
Substrate Type Beta-Gal Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate Galacton-Star™
Assay β-Gal (lacZ) Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer
Product Type β-Galactosidase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line Galacto-Star, NovaBright
9

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

ENCOMPASS_PREFERRED
Substrate CSPD™
Substrate Properties Chemical Substrate
Content And Storage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format 96-well Plate
For Use With (Equipment) Luminometer
Product Type Phosphatase Assay
Target Enzyme Placental Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility High-throughput Compatible
Product Line NovaBright
Detection Method Chemiluminescence
10

Invitrogen™ Phospha-Light™ SEAP Reporter Gene Assay System

Chemiluminescent reporter gene assay for sensitive detection of secreted human placental alkaline phosphatase used as a reporter protein.

ENCOMPASS_PREFERRED
Content And Storage This kit contains the substrate, required buffers, enhancer, and control Placental Alkaline Phosphatase.
Store the product at 4°C.
Form Liquid
Readout End Point
Compatible Cells Mammalian Cells
Target Enzyme Alkaline Phosphatase
Substrate Type AP (Alkaline Phosphatase) Substrate
For Use With (Application) Enzyme Assay
High-throughput Compatibility High-throughput Compatible
Detection Method Chemiluminescence
Substrate CSPD™
Assay Reporter Assay
Substrate Properties Chemical Substrate
For Use With (Equipment) Luminometer
Product Type Secreted Human Placental Alkaline Phosphatase Reporter Gene Assay System
Sample Type Cell Cultures
Label or Dye Unconjugated
Product Line NovaBright, Phospha-Light
11

Invitrogen™ BLOCK-iT™ Lentiviral Pol II miR RNAi Expression System

Combines Invitrogen's BLOCK-iT Pol II miR RNAi and ViraPower™ Lentiviral technologies to facilitate creation of a replication-incompetent lentivirus that delivers a microRNA (miRNA) sequence of interest to dividing or non-dividing mammalian cells for RNA interference (RNAi) analysis

ENCOMPASS_PREFERRED
12

Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit

For lentiviral delivery and genomic integration of DNA coding for shRNA

ENCOMPASS_PREFERRED
13

Invitrogen™ BLOCK-iT™ Lentiviral Pol II miR RNAi Expression System with EmGFP

Combines Invitrogen's BLOCK-iT Pol II miR RNAi and ViraPower™ Lentiviral technologies to facilitate creation of a replication-incompetent lentivirus that delivers a microRNA (miRNA) sequence of interest to dividing or non-dividing mammalian cells for RNA interference (RNAi) analysis

ENCOMPASS_PREFERRED
14

Invitrogen™ BLOCK-iT™ Pol II miR RNAi Expression Vector Kit with EmGFP

Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript

ENCOMPASS_PREFERRED
15

Invitrogen™ BLOCK-iT™ Pol II miR RNAi Expression Vector Kit

Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript

ENCOMPASS_PREFERRED