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Protein Labeling Reagents

Various reagents designed to selectively label target proteins. Products include classic dyes, radioisotope labels, biotinylation reagents, fluorescent labels, molecular probes, assays, and labeling kits.
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1
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Cytiva Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye 5-packs

More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye packs are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.

Content And Storage 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.
Kit Contents Five vials of dye
Solubility Soluble in cold water and hot water
Sufficient For Labeling 1mg Protein
Quantity 5 vials each sufficient to label 1 mg protein
2
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Cytiva Cy3 NHS Ester Mono-Reactive CyDye

Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.

Kit Contents Foil packs of pre-measured dried dye
Format Dried
pH Range 3 to 10
Product Type Cy3 NHS Ester
Color Red
Label or Dye Cy3
3
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Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Maleimide)
GSA_VA

For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides

Kit Contents Foil packs, each containing 1, 5 or 25mg dried dye
pH Range 3 to 10
4
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Cytiva Amersham™ CyDye™ Value Packs (bis-Reactive NHS Ester)
GSA_VA

Suitable for general labeling of amine residues

Kit Contents Foil packs, each containing 5, 10 or 50mg dried dye
5
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Cytiva CypHer5E NHS Esters

A red-excitable, pH-sensitive cyanine dye derivative, reporting receptors movement from the cell surface into acidic endosomes upon agonist stimulation.

Product Type CypHer5E NHS Ester
Formulation 100% CypHer 5E
Color Blue
Solubility Easily soluble in methanol; Very slightly soluble in cold water and hot water.
Emission 663
For Use With (Application) This reagent has been shown to be suitable for live cell receptor internalization studies using the IN Cell Analyzer 3000, IN Cell Analyzer 1000, and confocal microscopes. 0.5 mL of stock reagent will be sufficient for up to 500 wells.
6

Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye

Use for highly accurate labeling of amine groups. Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.

Kit Contents Foil packs of premeasured dye
Format Powdered
pH Range 3 to 10
Product Type Cy5.5 NHS Ester
Color Blue
Label or Dye Cy5.5
7
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Cytiva Amersham CyDye Value Packs (bis-Reactive NHS Ester), Purple
GSA_VA

Label proteins, antibodies and more. Cytiva Amersham CyDye Value Packs (bis-Reactive NHS Ester) provide maximum economy in general labeling applications. Available in a variety of reactive dye colors: red (Cy3), blue (Cy5.5) and purple (Cy7).

8
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Cytiva CyDye™ DIGE Fluors: Cy2

Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel

Content And Storage -15°C to -30°C
Format Solid
Product Type Amersham CyDye DIGE Fluor Cy2 Minimal Dye
Color Blue
For Use With (Application) For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
9
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Cytiva CyDye™ DIGE Fluors: Cy5

Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel

Content And Storage -15°C to -30°C
Format Solid
Product Type Amersham CyDye DIGE Fluor Cy5 Minimal Dye
Color Red
For Use With (Application) For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
10
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Cytiva CyDye™ DIGE Fluor Minimal Dye Labeling Kits

Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. Amersham CyDye™ DIGE Fluor Minimal Labeling Kits contain size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.

Content And Storage -15°C to -30°C
Format Solid
Color Blue, Green, Red
11
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Cytiva DeStreak Rehydration Solution

Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.

Content And Storage 2°C to 8°C
Kit Contents Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent
pH Range >7
Product Type Destreak Reagent
For Use With (Application) Improves reproducibility and quality of 2D Gels by preventing streaking
12
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Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes

Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.

Format Solid
Product Type Cy3B NHS Ester
Molecular Weight (g/mol) 771
Formulation 100% Cy3B (NHS ester)
Color Red
Solubility Soluble in aqueous buffers
Emission 570
Purity >99%
Excitation 559
13
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Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Hydrazide)

For the labeling of free carbonyl groups of glycoproteins and carbohydrates

Content And Storage 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.
pH Range 3 to 10
Solubility Soluble in cold water and hot water
For Use With (Application) For labelling via carbonyl groups. For use in steady state fluorescence, FP and FRET applications
14
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Cytiva Amersham™ CyDye™ Post-Labeling Reactive Dye Packs

Reactive CyDye fluors specifically optimized for nucleic acid microarray labeling. Cytiva CyDye Post-Labelling Reactive Dye Pack provides conveniently packaged, highly reactive Cy3 and Cy5 monofunctional NHS ester to label probes via the post-labeling (amino allyl) route.

15
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Cytiva CyDye™ DIGE Fluors: Cy3

Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel

Content And Storage -15°C to -30°C
Format Solid
Product Type Amersham CyDye DIGE Fluor Cy3 Minimal Dye
Color Green
For Use With (Application) For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel