Crosslinking Reagents
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Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO
EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach.
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Cell Signaling Technology CAR (Whitlow/218 Linker) Cell Enrichment Kit, Immunoaffinity, 1 Kit
The CAR (Whitlow/218 Linker) Cell Enrichment Kit is designed to positively select cells engineered to express a Whitlow/218 linker-containing single-chain variable fragment (scFv)-based chimeric antigen receptor (CAR) on the cell surface. This kit uses an indirect magnetic bead-based immunoaffinity enrichment protocol to yield highly pure, viable, and bead-free CAR positive cells from complex, heterogeneous cell populations.
- Enables positive selection of cells expressing scFv-based CARs with a Whitlow/218 linker.
- Produces highly pure, viable cells that are free of magnetic beads after processing.
- Utilizes paramagnetic polystyrene beads (4.5 μm diameter) coupled to streptavidin via a DNase I-cleavable DNA linker.
- Facilitates downstream analyses such as flow cytometry and single-cell omics assays.
- Features a column-free isolation method using a magnet.
