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Crosslinking, Labeling and Protein Modification

A variety of reagents used to modify proteins for identification, analysis, research, and other applications.
Protein Modification Reagents (274)
Protein Labeling Reagents (170)
Crosslinking Reagents (121)
Tandem Mass Tag Reagents (51)
Protein and Antibody Labeling Kits (33)

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Bolton-Hunter Reagent, Thermo Scientific Chemicals

CAS: 34071-95-9 Molecular Formula: C13H13NO5 Synonym: N-Succinimidyl-3-(4-hydroxyphenyl)propionate; Rudinger reagent

CAS 34071-95-9
Synonym N-Succinimidyl-3-(4-hydroxyphenyl)propionate; Rudinger reagent
Molecular Formula C13H13NO5
2

Thermo Scientific™ iodoTMT Isobaric Label Reagents and Kits
GSA_VA

Use Iodoacetyl Tandem Mass Tag (iodoTMT) Isobaric Label Reagents to label sulfhydryl-containing peptides for the multiplexed detection and quantitation of cysteine modification using tandem mass spectrometry analysis.

Workflow Step Protein Labeling
Final Product Type Protein
For Use With (Equipment) Mass Spectrometer
Label or Dye Tandem Mass Tag™ (TMT) Labels
Starting Material Cell Lysate
Detection Method Mass Spectrometry
3

Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

EDGE
4

Thermo Scientific™ Pierce™ Alkylating Reagents

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

EDGE
Product Type Alkylating Reagent
Product Line Pierce
5

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
GSA_VA

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

EDGE
Cell Permeability Cell-Impermeant
Product Type Sulfo-NHS-LC-Biotin
Spacer Mid-length
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
6

Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

EDGE
Shipping Condition Wet Ice
Content And Storage Upon receipt store desiccated at -20°C.
Description EDC
Cleavable No
Water Soluble Yes
Form Powder
Molecular Weight (g/mol) 191.7
Solubility Water
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Chemical Reactivity Amine-Carboxyl
Cell Permeability No
Format Standard, Single-use, Premium-grade
Product Type Crosslinker
Spacer Short (<10 Å)
Reactive Moiety Carbodiimide
Spacer Arm Length 0.0 Å
PEGylated No
7

Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
GSA_VA

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

EDGE
Molecular Weight (g/mol) 217.13
Solubility Water
PEGylated No
8

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin
GSA_VA

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

EDGE
Cell Permeability Cell-Impermeant
Product Type Sulfo-NHS-Biotin
Spacer Short
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
9

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

EDGE
Cleavable No
Product Type Crosslinker
Molecular Weight (g/mol) 572.43
Solubility Water
PEGylated No
10

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin

Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

EDGE
Cell Permeability Cell-Impermeant
Product Type NHS-PEG4-Biotin
Label or Dye Biotin
Solubility DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water
Chemical Reactivity Amine
Product Line EZ-Link
11

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format
GSA_VA

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

EDGE
Form Solid
12

Thermo Scientific™ Pierce™ Sodium meta-Periodate

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).

EDGE
13

Thermo Scientific™ EZ-Link™ Sulfo NHS-LC-LC-Biotin, No-Weigh™ Format

Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin enables simple and efficient biotin labeling of antibodies, proteins, and any other primary amine–containing macromolecules.

EDGE
Label Type Biotin & Analogs
14

Thermo Scientific™ Guanidine-HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

EDGE
15

Thermo Scientific™ Pierce™ Fluorescence Biotin Quantitation Kit

Determine biotinylation levels in labeled antibodies and other proteins using this fluorescent assay reagent and HABA dye for rapid and sensitive measurement.

EDGE