Crosslinking, Labeling and Protein Modification
Invitrogen™ Click-iT™ sDIBO Alkynes for copper-free click chemistry
Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor™, and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.
Invitrogen™ Click-iT™ Protein Enrichment Kit, for click chemistry capture of azide-modified proteins
Capture of click-chemistry-based azide-modified proteins
Invitrogen™ Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit
Click-iT™ HPG Alexa Fluor™ 594 Protein Synthesis Assay Kit
STEMCELL Technologies Collagenase C, ACF, Size: 1 g
Collagenase C, Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials. Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases including: collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Collagenase C, ACF contains low levels of tryptic activity, similar to Collagenase Type IV, and is intended for use in applications where it is necessary to prevent the introduction of potential animal-derived pathogens.
STEMCELL Technologies Collagenase B, ACF, Size: 1 g
Collagenase B, Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials. Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Collagenase B, ACF contains higher collagenase and caseinase levels than Collagenase A and is intended for use in applications where it is necessary to prevent the introduction of potential animal-derived pathogens.
STEMCELL Technologies Collagenase B, ACF, Size: 100 mg
Collagenase B, Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials. Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Collagenase B, ACF contains higher collagenase and caseinase levels than Collagenase A and is intended for use in applications where it is necessary to prevent the introduction of potential animal-derived pathogens.
STEMCELL Technologies Collagenase A, ACF, Size: 100 mg
Collagenase A, Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials. Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. Collagenase is capable of digesting native collagen fibrils commonly found in connective tissues and therefore is frequently used for tissue dissociation. Collagenase preparations contain the activity of several proteases, including collagenase, caseinase, clostripain, and trypsin (Kessler & Yaron). Collagenase A, ACF contains levels of proteolytic activity similar to type I and type II collagenases. Collagenase A, ACF is intended for use in applications where it is necessary to prevent the introduction of potential animal-derived pathogens.
Cell Signaling Technology DyLight™ 488 Phalloidin, Lyophilized Solid, 300 Units
DyLight™ 488 Phalloidin is a fluorescent probe designed for labeling the cytoskeleton of fixed cells by binding with high affinity to F-actin. Due to the inherent toxicity of phalloidin, this product is intended for use in fixed cells only and is not suitable for live cell imaging. The lyophilized material, when reconstituted, provides sufficient stock solution for 3,000 assays.
- Excitation: 495 nm; Emission: 521 nm.
- Stable for 1 year at -20°C when stored desiccated and protected from light.
- Reconstitution requires anhydrous DMSO; aqueous solution stability is low.
- Compatible with immunofluorescence in all species.
- Recommended for use with methanol-free 4% formaldehyde fixation.
Cell Signaling Technology Alexa Fluor™ 647 Phalloidin, Lyophilized, 300 Units
Alexa Fluor™ 647 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is designed for use on fixed and permeabilized samples. Reconstituted in anhydrous DMSO, the stock solution provides enough material to perform 200 assays at a 1:100 dilution and 100 μl assay volume.
- Excitation and emission wavelengths of 650 nm and 668 nm, respectively.
- Provided as a lyophilized solid stable for 1 year at -20°C when stored desiccated and protected from light.
- Validated for immunofluorescence applications including frozen and immunocytochemistry samples.
- High affinity binding to F-actin for cytoskeletal visualization.
- Compatible with all species.
Cell Signaling Technology DyLight™ 594 Phalloidin, 300 Units
DyLight™ 594 Phalloidin allows researchers to fluorescently label the cytoskeleton of fixed cells through the high-affinity binding of phalloidin to F-actin. This product is provided as a lyophilized solid and is intended for use on fixed cells only due to the toxicity associated with phalloidin. The reconstituted stock solution provides enough material to perform 900 assays based on a 1:300 dilution and a 100 μl assay volume.
- Fluorescent properties include excitation at 585 nm and emission at 613 nm.
- Validated for use in immunofluorescence applications including frozen and immunocytochemistry samples.
- Stable for 1 year when stored at -20°C in lyophilized form or after reconstitution in anhydrous DMSO.
- Compatible with all species.
Cell Signaling Technology Alexa Fluor™ 555 Phalloidin, F-Actin Staining, 300 Units
Alexa Fluor™ 555 Phalloidin is a fluorescent stain used to label the cytoskeleton by binding to F-actin. Due to the inherent toxicity of phalloidin, this product is specifically intended for use in fixed and permeabilized cellular samples. Following reconstitution in anhydrous DMSO, a single vial provides sufficient material for 100 assays based on a 1:100 dilution and a 100 μl assay volume.
- Excitation: 555 nm; Emission: 565 nm.
- Stable for 1 year at -20°C when stored as a lyophilized solid or once reconstituted in anhydrous DMSO.
- Suitable for immunofluorescence applications including immunocytochemistry and frozen sections.
- Cross-reactivity observed across all species.
Cell Signaling Technology DyLight™ 554 Phalloidin, Cytoskeleton Labeling, 300 Units
DyLight™ 554 Phalloidin is a fluorescent probe designed for labeling F-actin in fixed cells. It utilizes a high-affinity interaction with F-actin to provide clear visualization of the cytoskeleton in various immunofluorescence applications. This product is supplied as a lyophilized solid and requires reconstitution in anhydrous DMSO before use.
- Excitation and emission wavelengths of 551 nm and 572 nm, respectively
- Stable for 1 year when stored at -20°C, desiccated and protected from light
- Suitable for immunofluorescence in fixed cells and frozen tissue sections
- Provides enough material for 3,000 assays when used at a 1:400 dilution in a 100 μl volume
- Recommended for use with anhydrous DMSO reconstitution only
Cell Signaling Technology p38 MAPK Control Cell Extracts, 2-Piece Set
These control cell extracts are designed for use in Western blotting to provide negative and positive control samples for p38 MAPK analysis. The set includes total cell extracts from C-6 glioma cells, providing both nonphosphorylated control material and phosphorylated samples treated with Anisomycin to induce p38 MAPK activation. These extracts are supplied in SDS sample buffer, ready for gel electrophoresis applications.
- Includes nonphosphorylated C-6 cell extract as a negative control.
- Includes phosphorylated C-6 cell extract treated with Anisomycin as a positive control.
- Optimized for Western blotting applications.
- Supplied in SDS sample buffer (62.5 mM Tris-HCl, pH 6.8 at 25°C, 2% w/v SDS, 10% glycerol, 50 mM DTT, and 0.01% w/v phenol red or bromophenol blue).
- Requires boiling for 3 minutes before use; load 15 μl per lane.