Competent Cells
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Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | High Copy Plasmid |
| Reduces Recombination | Yes (recA1) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Chemically Competent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | No |
Invitrogen™ One Shot™ Stbl3™ Chemically Competent E. coli
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli Cells, 96-well plate
MultiShot TOP10 Chemically Competent E. coli Cells provide high-efficiency transformation for routine cloning in an automation-ready 96-well format. Ideal for stable propagation of high-copy plasmids and blue/white screening in high-throughput workflows.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli
One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ BL21(DE3) Chemically Competent E. coli Cells, FlexPlate
MultiShot BL21(DE3) Chemically Competent E. coli Cells offer high-efficiency protein expression in a flexible 96-well format. Breakable into 8-well strips, it enables convenient, scalable workflows for recombinant protein production and parallel cloning experiments.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ Stbl3™ Chemically Competent E. coli Cells, 96-well plate
MultiShot Stbl3 Chemically Competent E. coli Cells support cloning of unstable vectors with direct repeats. Automation-compatible 96-well format ensures high-throughput use while the Stbl3 strain minimizes recombination and maintains complex constructs.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli
The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ Mach1™ T1R Chemically Competent E. coli Cells, 96-well plate
MultiShot Mach1™ T1R Chemically Competent E. coli Cells enable rapid, high-efficiency cloning in an automation-ready 96-well format. Fast-growing and phage-resistant, these cells support same-day transformation and streamlined high-throughput workflows.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ BL21(DE3) Chemically Competent E. coli Cells, 96-well plate
MultiShot BL21(DE3) Chemically Competent E. coli Cells enable high-throughput protein expression with compatibility for automated liquid handling. This ready-to-use format streamlines cloning and expression workflows using the reliable BL21(DE3) expression strain.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ DH5α T1R Chemically Competent E. coli Cells, 96-well plate
MultiShot DH5α T1R Chemically Competent E. coli Cells provide reliable, high-efficiency cloning in an automation-friendly 96-well format. Ideal for high-throughput DNA manipulation and plasmid propagation using the trusted DH5α T1R strain.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21(DE3) Chemically Competent E. coli
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ One Shot™ BL21-AI™ Chemically Competent E. coli
One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli
MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
| High-throughput Compatibility | High |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | High Copy Plasmid |
| Reduces Recombination | Yes (recA1) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Product Type | Chemically Competent Cells |
| Product Line | MultiShot |
| Contains F' Episome | No |
Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli
One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | Not suitable for cloning unstable DNA |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Promoter | Trc, Tac, Lac |
| Preparing Unmethylated DNA | No |
| Reduces Recombination | Yes (recA1) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptamycin, Tetracycline) |
| Format | Tube |
| Product Type | Chemically Competent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | Yes |