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Reaction buffers for use in experiments involving restriction enzymes, such as restriction digestions. Different buffer solutions create optimal reaction conditions for specific restriction enzymes, or facilitate multiple enzymatic reactions.
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3-D Life 10x CB Buffer (pH 7 2) Phenol Red-Free (600 l) Hydrogel Component DESCRIPTION 3-D Life 10x CB-Buffer (pH 7 2) Phenol Red-Free is a component of the 3-D Life Hydrogel system In order to avoid interferences with light microscopy analysis this buffer does not contain Phenol Red It is a 10x concentrated salt and buffer solution which is diluted to physiological strength when used in the 3-D Life Hydrogel system It is used for the formation of slow gelling 3-D Life Hydrogels where it provides a cell compatible environment at pH 7 2 In conjunction with 3-D Life 10x CB-Buffer (pH 5 5) it is used to generate pHs of intermediate values to modify the gelation kinetics of fast gelling gels APPLICATION Used with other components of the 3-D Life Hydrogel System to establish 3D cell cultures QUANTITY 600 l (sufficient for at least 6 ml 3-D Life Hydrogel)
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