Neuroscience Reagents and Kits
Gibco™ PSC Dopaminergic Neuron Differentiation Kit
The Gibco™ PSC Dopaminergic Neuron Differentiation Kit is a culture media system that enables the differentiation of human pluripotent stem cells (hPSCs) into functional midbrain dopaminergic neurons.
Cell Signaling Technology PathScan™ Nestin Sandwich ELISA Kit II, Human, 960 Assays
The PathScan™ Nestin Sandwich ELISA Kit II is a solid phase sandwich enzyme-linked immunosorbent assay that detects endogenous levels of nestin protein. The assay utilizes microwells coated with a nestin mouse monoclonal antibody to capture the protein from cell lysates, followed by detection with a nestin rabbit monoclonal antibody and an HRP-linked anti-rabbit IgG secondary antibody. The colorimetric readout, developed using TMB substrate, is proportional to the quantity of nestin protein present.
- Validated for detection of endogenous nestin protein
- Includes ready-to-use reagents such as detection antibody diluent, HRP diluent, and wash buffer
- Provides a customizable 96-well strip plate format for experimental flexibility
- Compatible with spectrophotometric determination at 450 nm
- Designed for research use only
Cell Signaling Technology PathScan™ Nestin Sandwich ELISA Kit II, Human, 5 Kit
The PathScan™ Nestin Sandwich ELISA Kit II is a solid phase sandwich enzyme-linked immunosorbent assay that detects endogenous levels of nestin protein. The kit utilizes a nestin mouse monoclonal antibody coated onto microwells to capture the target protein from cell lysates. Detection is achieved using a nestin rabbit monoclonal antibody, followed by an HRP-linked anti-rabbit IgG antibody and TMB substrate to produce a colorimetric signal proportional to the amount of nestin present.
- Validated for detection of human nestin protein.
- Includes pre-coated 96-well strip plates for flexible configuration.
- Uses an HRP-based detection system with a colorimetric output measured at 450 nm.
- Supplied with all necessary buffers, diluents, and stop solution for assay execution.
- Storage at +4°C required for most components; Cell Lysis Buffer should be stored at -20°C.