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Secondary antibodies where goat is the host species. Secondary antibodies must be raised against the host species of the primary antibody. Includes different antigens, classifications, conjugations, and isotypes.
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
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Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. urity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
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This HRP-conjugated F(ab')₂ fragment goat anti-human IgG (H+L) secondary antibody is for in vitro research. It reacts with whole molecule human IgG and light chains of other human immunoglobulins. F(ab')₂ fragments avoid binding to Fc receptors on live cells or to Protein A/G.
Target: Human
Host: Goat
Antibody format: F(ab')₂ Fragment
Specificity: IgG (H+L)
Conjugate: HRP
Clonality: Polyclonal
Purity: Purified via pepsin digestion and immunoaffinity chromatography.
Usage: Immunohistochemistry, Western blotting, and ELISA.
Reduced background for mammalian cell immunostaining.
Physical state: Freeze-dried solid.
Storage (freeze-dried): 2–8°C.
Working concentration: 1:500 to 1:200,000 across applications.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More