Protein Phosphorylation Antibody Pairs
Gibco™ Floor Plate Cell Expansion Kit
The Floor Plate Cell Expansion Kit is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit that is also offered separately.
Cell Signaling Technology Phospho-SMAD2 (Ser465/467) Matched Antibody Pair, Human, Mouse, Monkey, 1 Kit
The Phospho-SMAD2 (Ser465/467) Matched Antibody Pair is designed for use in immunoassay technologies and high-throughput ELISA platforms that require specialized or custom antibody labeling, such as fluorophores, lanthanides, biotin, or beads. This antibody pair includes capture and detection antibodies that bind to non-overlapping epitopes. It is compatible with platforms including MSD, Quanterix Simoa, Luminex, and Alpha Technology (AlphaScreen, AlphaLISA, LANCE, HTRF).
- Supplied as a kit containing 100 μg each of mouse monoclonal antibody and rabbit monoclonal antibody
- Formulated in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, 140 mM NaCl)
- BSA and azide free for easy labeling
- Validated for reactivity in human, mouse, and monkey species
- Ideal for development of high-throughput ELISA assays
Cell Signaling Technology Phospho-SMAD2 Monoclonal Antibody, Rabbit, Human, 100 μg
This rabbit monoclonal antibody specifically detects endogenous levels of SMAD2 only when dually phosphorylated at serines 465 and 467. The antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human SMAD2. This carrier-free formulation is ideal for custom antibody labeling technologies, including conjugation to fluorophores, metals, lanthanides, and oligonucleotides.
- Specifically detects SMAD2 phosphorylated at Ser465 and 467
- BSA and azide free for versatile downstream labeling applications
- Quality control tested by size exclusion chromatography to ensure antibody integrity
- Validated for use in ELISA-like assays
- Recombinant rabbit monoclonal antibody
Cell Signaling Technology Phospho-Stat5a (Tyr694) Matched Antibody Pair, Human, Unconjugated, 1 Kit
The Phospho-Stat5a (Tyr694) Matched Antibody Pair is designed for use in immunoassays and high-throughput ELISA platforms that require custom antibody labeling. This kit includes capture and detection antibodies that bind to non-overlapping epitopes, allowing for precise detection of phosphorylated Stat5a (Tyr694) without cross-reactivity with phospho-Stat5b (Tyr699).
- Compatible with various conjugation methods including fluorophores, lanthanides, biotin, and beads.
- Suitable for platforms such as MSD, Quanterix Simoa, Alpha Technology, and Luminex.
- Supplied as BSA and azide free for optimal performance in downstream labeling.
- Validated for human species reactivity.
- Contains 100 μg each of capture and detection monoclonal antibodies.
- Storage at -20°C recommended in single-use aliquots.
Cell Signaling Technology FastScan™ Phospho-SMAD2 ELISA Kit, Human, Mouse, Monkey, 5 Kit
The FastScan™ Phospho-SMAD2 (Ser465/467) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed to detect endogenous levels of SMAD2 when phosphorylated at Ser465/467. The assay functions by incubating samples with a capture antibody and an HRP-linked detection antibody, forming a complex immobilized to the plate via an anti-tag antibody. The intensity of the signal generated after TMB substrate addition is proportional to the concentration of phospho-SMAD2 (Ser465/467) in the sample.
- Provides high-sensitivity detection of phosphorylated SMAD2 (Ser465/467).
- Utilizes a sandwich ELISA format for reliable and specific protein quantification.
- Validated for use with human, mouse, and monkey samples.
- Includes all necessary reagents for a complete assay workflow.
- Enables custom plate configurations with included strip-well format.
Cell Signaling Technology PathScan™ Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit, Human, Mouse, Mink, 20 Kit
The PathScan™ Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay that detects endogenous levels of SMAD2 when phosphorylated at Ser465/467. This kit utilizes a SMAD2 mouse monoclonal antibody coated onto microwells to capture both phospho and non-phospho SMAD2 from cell lysates. A phospho-specific detection antibody and an HRP-linked secondary antibody are subsequently used to quantify phosphorylation levels via colorimetric substrate development.
- Provides reliable detection of endogenous SMAD2 phosphorylation at Ser465/467
- Validated for use with human, mouse, and mink samples
- Includes all necessary components for colorimetric detection in a 96-well format
- Designed for research use only
Cell Signaling Technology PhosphoPlus™ c-Myc (Ser62) Antibody Duet, Unconjugated, 1 Kit
The PhosphoPlus™ c-Myc (Ser62) Antibody Duet provides a targeted solution for evaluating the activation status of the c-Myc protein. Each kit contains an activation-state specific antibody alongside a total protein antibody, allowing for the simultaneous assessment of phosphorylated c-Myc (Ser62) and total c-Myc levels in research samples.
- Includes 100 μL each of rabbit monoclonal antibodies
- Validated for western blotting, immunofluorescence, chromatin IP-seq, flow cytometry, and immunoprecipitation
- Target reactivity includes human, mouse, and rat species
- Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, 50% glycerol and less than 0.02% sodium azide
- Recommended storage at -20°C without aliquoting
Cell Signaling Technology PathScan™ Phospho-Akt1 Chemiluminescent ELISA Kit, 50 Kit
The PathScan™ Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay designed to detect endogenous levels of phospho-Akt1 (Ser473) protein with a chemiluminescent readout. Utilizing low-volume microplates, this assay provides increased signal sensitivity and dynamic range while requiring smaller sample volumes compared to conventional chromogenic detection methods.
- Detects phospho-Akt1 (Ser473) in human, mouse, and rat cells.
- Features higher sensitivity and wider dynamic range than standard chromogenic ELISA.
- Requires only 50 μL of sample or reagents per microwell.
- Includes antibody-coated microwells for specific capture of phospho-Akt (Ser473).
- Supplied in a 12 8-well strip format for flexible configuration.
- Validated for research applications only.
Cell Signaling Technology PathScan™ Phospho-Akt1 Chemiluminescent Sandwich ELISA Kit, 10 Kit
The PathScan™ Phospho-Akt1 (Ser473) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) designed to detect endogenous levels of phospho-Akt1 (Ser473) protein. This kit utilizes a chemiluminescent readout, which typically offers a wider dynamic range and higher sensitivity compared to conventional chromogenic detection methods. It is provided in low volume microplates, allowing for increased sensitivity while using smaller sample volumes.
- Provides a chemiluminescent readout proportional to the quantity of phospho-Akt1 (Ser473) protein
- Detects endogenous levels of phospho-Akt1 (Ser473) in human, mouse, and rat samples
- Offered in low volume microplate format for enhanced sensitivity with smaller samples
- Custom antibody formulations specific to the kit
- Validated for research use only
Cell Signaling Technology FastScan Phospho-Akt1 ELISA Kit, Ser473, Human/Mouse/Rat/Monkey, 5 Kit
The FastScan™ Phospho-Akt1 (Ser473) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed to detect endogenous levels of Akt1 when phosphorylated at Ser473. The assay functions by incubating samples with a proprietary tagged capture antibody and an HRP-linked detection antibody, forming a sandwich with the target protein that is immobilized on the plate via an anti-tag antibody. The intensity of the signal measured at 450 nm is proportional to the amount of phospho-Akt1 (Ser473) present in the sample.
- Validated for use with human, mouse, rat, and monkey samples.
- Compatible with plate shakers for efficient incubation.
- Includes a positive control for assay validation.
- Custom antibody formulations ensure high specificity and sensitivity.
- Supports rapid analysis with TMB substrate and colorimetric detection.
Cell Signaling Technology PathScan™ RP Phospho-Stat5a ELISA Kit, Sandwich, Human, 10 Kit
The PathScan™ RP Phospho-Stat5a (Tyr694) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay designed to detect endogenous levels of Stat5a protein phosphorylated at Tyr694. This rapid protocol kit significantly reduces assay time to 1.5 hours by allowing the incubation of cell lysates and detection antibody on the coated microwell plate in a single step.
- Detects endogenous levels of Stat5a protein phosphorylated at Tyr694.
- Features a rapid 1.5-hour assay protocol.
- Utilizes a single-step sandwich formation for increased efficiency.
- Quantitative analysis based on absorbance proportional to phospho-Stat5a concentration.
- Validated for human species reactivity.
Cell Signaling Technology PathScan™ Phospho-SMAD2 Sandwich ELISA Kit, Human, Mouse, Mink, 5 Kit
The PathScan™ Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay that detects endogenous levels of SMAD2 when phosphorylated at Ser465/467. The kit utilizes a SMAD2 mouse monoclonal antibody coated onto microwells to capture SMAD2 protein from cell lysates. A phospho-specific detection antibody is subsequently used to identify serine phosphorylation, followed by an HRP-linked secondary antibody for colorimetric detection using TMB substrate.
- Quantitative detection of SMAD2 phosphorylated at Ser465/467
- Validated for use in human, mouse, and mink samples
- Includes all necessary reagents for sandwich ELISA
- Colorimetric detection method
- Compatible with cell lysates
Cell Signaling Technology PathScan™ RP Phospho-Stat5a Sandwich ELISA Kit, Human, 480 Assays
The PathScan™ RP Phospho-Stat5a (Tyr694) Sandwich ELISA Kit is a solid-phase enzyme-linked immunosorbent assay designed to detect endogenous levels of Stat5a protein phosphorylated at Tyr694. This rapid protocol kit streamlines the assay process, allowing for the detection of phosphorylated protein in 1.5 hours. By using a single-step incubation of cell lysates and detection antibody on a coated microwell plate, this assay provides a precise measurement where absorbance is proportional to the target phospho-protein quantity.
- Detects endogenous levels of Stat5a phosphorylated at Tyr694
- Reduced 1.5 hour assay time
- Solid-phase sandwich ELISA format
- High sensitivity with no cross-reactivity with phospho-Stat5b (Tyr699)
- Species reactivity: Human
Cell Signaling Technology PathScan Phospho-SMAD2 and SMAD3 Sandwich ELISA Kit, 50 x 96 Tests
The PathScan™ Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay that recognizes endogenous levels of phospho-SMAD2 (Ser465/467) and SMAD3 (Ser423/425) proteins. The assay utilizes microwells coated with a SMAD2/3 mouse monoclonal antibody to capture proteins from cell lysates. A phospho-specific detection antibody is subsequently used to identify captured targets, followed by an HRP-linked anti-rabbit IgG antibody and a colorimetric TMB substrate. The resulting color intensity is directly proportional to the amount of captured phospho-SMAD2 and phospho-SMAD3.
- Provides a sensitive method for detecting endogenous phospho-SMAD2 and phospho-SMAD3.
- Compatible with human, mouse, and mink samples.
- Includes all necessary assay reagents.
- Validated for high specificity for the conserved carboxy-terminal SSXS motif.
Cell Signaling Technology PathScan™ Phospho-SMAD2/SMAD3 Sandwich ELISA Kit, Lyophilized, 5 x 96 Tests
The PathScan™ Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay designed to detect endogenous levels of phospho-SMAD2 (Ser465/467) and phospho-SMAD3 (Ser423/425) proteins. The kit utilizes microwells coated with a SMAD2/3 mouse antibody to capture proteins from cell lysates. A detection antibody is added to identify captured phospho-SMAD2 and phospho-SMAD3, followed by an HRP-linked anti-rabbit IgG antibody and TMB substrate to generate a colorimetric signal proportional to protein quantity.
- Detects phospho-SMAD2 (Ser465/467) and phospho-SMAD3 (Ser423/425) proteins
- Includes all reagents for colorimetric detection
- Validated for human, mouse, and mink species
- Supports sensitive detection of endogenous signaling molecules
- Compatible with standard plate-based experimental workflows