Protein Phosphorylation Antibody Pairs

AMID BIOSCIENCES Amid Biosciences, Human RAF1-RBD Protein, Recombinant, RAS Binding Domain, 0.1 mg

Human RAF1-RBD is supplied as a recombinant RAS binding domain in a 0.1 mg format for research on RAS effector engagement and MAPK pathway signaling. Applications include measurement of GTP/GDP bound ratio of Ras in vitro, quantitation of GTP-bound Ras from tissue and cell lysates, RAS/RAF binding studies, inhibitor screening for RAS/RAF interaction. N-terminal 6XHis-GST tag enables immobilization of RAF1-RBD and RBD-Ras complexes on Ni-NTA or glutathione resin columns. Technical specifications are included below.- Quantity - 0.1 mg- Gene - RAF1- Mutation / Variant - Ras-binding domain- Organism - Human- Tag - N-terminal 6XHis and GST dual affinity tag- Expression System - E. coli- Purity - greater than 80% by Coomassie staining- UniProt ID - P04049- Amino Acid Range - 1-149- Buffer - 20 mM HEPES, pH 7.5, 100 mM NaCl, 20% Glycerol- Storage - -80 C- Biotinylated - NoThis product is for research use only.