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Nitrotetrazolium Blue chloride (298-83-9) is a redox-sensitive tetrazolium derivative It serves as a substrate for cellular enzymes particularly dehydrogenases Upon reduction Nitrotetrazolium Blue chloride forms a blue-violet formazan precipitate enabling visualization of enzyme activities and metabolic status in cell-based assays Nitrotetrazolium Blue chloride exerts its biological activity primarily through enzymatic reduction by dehydrogenases It is frequently applied as a chromogenic substrate in combination with alkaline phosphatase for immunohistochemical staining and Western blot analyses Based on these properties Nitrotetrazolium Blue chloride holds research potential in protein expression assays histological investigations and enzyme activity detection
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