This affinity‑purified chicken antibody specifically recognizes hamster (CHO) Nidogen‑1, a key basement‑membrane glycoprotein involved in extracellular matrix organization, laminin binding, and structural stabilization of the pericellular environment. Its high specificity supports sensitive detection of Nidogen‑1 in CHO lysates, purified protein preparations, and host cell protein impurity panels. The avian host species minimizes background from mammalian samples, enabling clean performance in ELISA, Western blotting, and orthogonal analytical assays. Consistent purification ensures low nonspecific binding and reliable lot‑to‑lot behavior, making this reagent well suited for bioprocess monitoring, HCP characterization, and development of quantitative assays targeting CHO‑derived matrix proteins.