Western Blot Substrates and Substrate Kits
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents
Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Sigma Aldrich Fine Chemicals Biosciences Atto 488 Protein Labeling
This kit contains sufficient amounts of reactive dye buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Bio Rad Laboratories PRECISION PLUS PROTEIN UNSTAIN
Standards, Unstained; Bio-Rad; Precision Plus Protein; 1mL; 100 applications
National Diagnostics ProtoGlow ECL | 200mL
ProtoGlow ECL delivers the latest technology developed for enhanced chemiluminescent detection on Western blots. The unique chemistry of the ProtoGlow ECL system increases the sensitivity of Western blots by up to 20 fold. This allows the detection of proteins at lower abundance and/or the use of higher dilutions of primary and secondary antibodies, economizing on these expensive reagents.
Sigma Aldrich Fine Chemicals Biosciences Atto 488 azide1MG
Atto 488 is a superior fluorescence label with high molecular absorption (90.000) and quantum yield (0.80) as well as sufficient stokes shift. It is optimised for excitation with argon laser and is characterized by high photo stability.The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - Click Chemistry).
Abcam ECL Substrate Kit (High Sensitivity), 200ML
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band.
The product is subject to the following: Abcam Restricted Use Statement
Rockland Immunochemicals Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP - 18-8817-33
Mouse TrueBlot ULTRA has been reported for use in IP and WB. Mouse IgG TrueBlot ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution. Specific conditions for each protein and antibody combination should be specifically optimized by the end user. We recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.
Abcam ECL Substrate Kit (Ultra High Sensitivity), 100ML
Ultra High Sensitivity ECL Substrate Kit ab133409 is a horseradish peroxidase (HRP) substrate specially developed for Western blotting detection of very low abundance proteins with 1.
The product is subject to the following: Abcam Restricted Use Statement
FUJIFILM Biosciences SCALEVIEW S Trial Kit
SCALEVIEW-S Trial Kit consists of tissue clearing reagents used for ScaleS a tissue clearing method developed by Dr Atsushi Miyawaki and his colleagues This method uses solutions with mainly urea The tissue is made transparent and adjusted RI for microscopic observation by soaking in 6 kinds of solutions SCALEVIEW-S0 SCALEVIEW-S1 SCALEVIEW-S2 SCALEVIEW-S3 SCALEVIEW-S4 SCALEVIEW-SMt 2mm section can be made transparent in 1 day using the reagents The method can make a transparent tissue keeping the structure of proteins Therefore it is possible to observe the tissue expressing fluorescent proteins and to label by antibody and/or fluorescent dye after tissue clearing Its also applicable to not only mouse and rat brain but also human after dye The transparent tissue can be observed for electric microscope or confocal microscope
Cell Signaling Technology SignalFire™ ECL Reagent, Chemiluminescent Substrate, Western Blot, 50 mL
SignalFire™ ECL Reagent is an enhanced chemiluminescent substrate designed for the detection of picogram amounts of protein via western blot analysis. By utilizing horseradish peroxidase (HRP) to convert luminol in the presence of hydrogen peroxide, this reagent produces an excited intermediate dianion that emits light upon returning to its ground state. Compared to standard substrates, this system offers enhanced signal robustness and an extended output duration of up to 3 hours.
- Provides high sensitivity for detection of picogram levels of protein
- Features extended signal output for up to 3 hours
- Compatible with HRP-conjugated secondary antibodies
- Requires no further water dilution due to 2X concentrate formulation
- Stable for 36 months when stored at room temperature
Seracare Life Sciences Inc LUMIGLO RES HRP CHEMI SUBS
LumiGLO Reserve is a lumino-based chemiluminescent substrate designed for rapid and sensitive detection of peroxidase-labeled conjugates. This substrate provides maximum sensitivity in the femtogram range, making it ideal for applications where the protein of interest is in low concentrations. Given the high light intensity, images may be more quickly captured and multiple exposures easily obtained. The 2-component format is easily prepared and is provided with KPL's Wash Solution, futher enabling blots with excellent signal-to-noise.
Seracare Life Sciences Inc LUMIGLO RES HRP CHEMI SUBS
LumiGLO Reserve is a lumino-based chemiluminescent substrate designed for rapid and sensitive detection of peroxidase-labeled conjugates. This substrate provides maximum sensitivity in the femtogram range, making it ideal for applications where the protein of interest is in low concentrations. Given the high light intensity, images may be more quickly captured and multiple exposures easily obtained. The 2-component format is easily prepared and is provided with KPL's Wash Solution, futher enabling blots with excellent signal-to-noise.
Advansta Inc WESTERNBRIGHT ECL KIT, FOR 200
WesternBright ECL kit contains 200ml; sufficient for 2000cm2 membrane. Stronger signal than Amersham ECL or Pierce ECL. Detect low pg protein amounts. Use up to ten-times less antibody. WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. WesternBright ECL requires as little as ten times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures to be carried out without substantial signal decay.
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