Gel Electrophoresis Buffers and Diluents
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
Bioworld Tris-Glycine (TG) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine (TG) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris base and 0.192M Glycine. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated using bioPlus Tris powder to ensure exceptional purity along with the absence of nuclease and protease activities. TG Buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Bioworld Tris-Glycine (TG) Running Buffer, Ready-Pack (1X), 6 PK(s)
Tris-Glycine The TG Buffers are blended using pure reagents to provide the highest quality buffers for protein research. Ideal for protein separation, this buffer is formulated using bioPlus Tris powder to ensure exceptional purity and the absence of nuclease and protease activities. TG Buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes. Technical Information:Uniform white powderUltra Pure GradeContents (1X solution): 0.025M Tris base and 0.192M Glycine.pH @25C: 8.3Each Ready-pack prepares 1L of 10X Concentrate.Supplied as 1x20 PacksStorage: RTHazard: Irritant
Bioworld Tris-Glycine (TG) Running Buffer, Ready-Pack (1X), 1 PK(s)
Tris-Glycine The TG Buffers are blended using pure reagents to provide the highest quality buffers for protein research. Ideal for protein separation, this buffer is formulated using bioPlus Tris powder to ensure exceptional purity and the absence of nuclease and protease activities. TG Buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes. Technical Information:Uniform white powderUltra Pure GradeContents (1X solution): 0.025M Tris base and 0.192M Glycine.pH @25C: 8.3Each Ready-pack prepares 1L of 10X Concentrate.Supplied as 1x20 PacksStorage: RTHazard: Irritant
Biotium Blocking Buffers Background Reducers Fluorescence Microscopy Western and Protein Analysis Chemical Reagents 1/EA
This buffer is a component of the TrueBlack WB Blocking Kit It is available for customers who have evaluated the kit and prefer to use the Blocking Buffer for membrane blocking and antibody incubation steps For blocking and antibody dilution for 25 membranes (500 mL buffer 10 mL per membrane)
Sigma Aldrich Fine Chemicals Biosciences DILUENT C,GEN MEMBRN LABEL 6PK
Diluent C FOR General Membrane Labeling 6/PK
Bio Rad Laboratories READYPREP PROTEIN EXTRACTION K
Kit, Extraction Bio-Rad ReadyPrep Protein (Membrane I) 50 preps
Boston Bioproducts Inc Transfer Buffer (10X, Electro Blotting) - 4L
The 10X Transfer Buffer-Electro Blotting is the most commonly used buffer in the wet transfer system for the electrotransfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. The addition of 10% methanol in thhis buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
Boston Bioproducts Inc NP-40 Lysis Buffer - 250ml
The NP-40 Lysis Buffer is a solubilizing agent used to prepare cell extracts for immunoassays and blotting techniques. As one of the most popular lysis buffers on the market, the NP-40 lysis buffers can support a variety of laboratory workflows. Relying on the non-ionic detergent NP-40 as its primary solubilizing agent, this Lysis buffer is weaker than a RIPA buffer, and can permeabilize cell membranes, partially open the nucleus without fully disrupting it, and lyse mitochondiral membranes.
Boston Bioproducts Inc Transfer Buffer (10X, Electro Blotting) - 1L
The 10X Transfer Buffer-Electro Blotting is the most commonly used buffer in the wet transfer system for the electrotransfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. The addition of 10% methanol in thhis buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.
Boston Bioproducts Inc NP-40 Lysis Buffer (2X) - 250ml
The 2X NP-40 Lysis Buffer is a solubilizing agent used to prepare cell extracts for immunoassays and blotting techniques. As one of the most popular lysis buffers on the market, the NP-40 lysis buffers can support a variety of laboratory workflows. Relying on the non-ionic detergent NP-40 as its primary solubilizing agent, this Lysis buffer is weaker than a RIPA buffer, and can permeabilize cell membranes, partially open the nucleus without fully disrupting it, and lyse mitochondiral membranes.
Bioworld FastBlot™ Transfer Buffer pH 11.0 (5X), 500 mL
For electrotransfer of proteins to PVDF or nitrocellulose membranes. 1X Transfer Buffer contains 10 mM CHAPS pH 11 and 10% Methanol. Does not contain SDS.May be useful for large proteins or very basic proteins and for N-terminal sequencing applications. Commonly used with semi-dry transfer.pH (@ 25°C): 11
Surmodics Inc StabilGuard™ Immunoassay Stabilizer, 1L
StabilGuard™ Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications.
Quality Biological Inc RNA Gel Buffer (10X) (MOPS Buffer), 500 mL
MOPS Buffer is used at 1X for the electrophoresis of RNA as the running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels. MOPS buffer is also used for Northern Blots -the hybridization of total RNA or mRNA to a membrane. Liquid, 10X concentration
Filtered through a 0.22 µm membrane
DNase, RNase Tested
Applications: MOPS Buffer is suitable for use in RNA Gel Electrophoresis, Northern Blot Analysis, and when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase Tested
pH: 7.0 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Alkali Scientific Transfer/Electro Blotting 10X Solution, 1 Liter
Transfer/Electro Blotting 10X Solution is a high-quality buffer used for the electroblotting process in protein analysis. This 1L bottle of 10X solution can be diluted to create multiple liters of buffer, making it ideal for high-throughput applications such as Western blotting. The solution is formulated to provide optimal conditions for protein transfer from gels to membranes, ensuring efficient binding and minimal protein loss during the blotting process. It is particularly used in immunoblotting and protein detection, where high-quality protein transfer is critical for successful analysis and reproducible results.
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