Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (39,385)

Singleplex and Multiplex Bead Based Immunoassays (3,365)

ELISA Substrates and Reagents (459)

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Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

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Invitrogen™ Cell Lysis Buffer II

Cell Lysis Buffer II is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications.

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Invitrogen™ ProcartaPlex™ Cell Lysis Buffer

Used to lyse cells under nondenaturing conditions. Invitrogen™ ProcartaPlex™ Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.

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Invitrogen™ Human Interferon alpha (Cell culture) ELISA Kit

ELISA

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Invitrogen™ ProcartaPlex™ Human Cell Death Panel, 4plex

The ProcartaPlex Human Cell Death Panel 4plex enables cancer research by analyzing four protein targets involved in cell death using Luminex xMAP technology.

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Invitrogen™ Human Interferon alpha (Cell culture) ELISA Kit

ELISA

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R&D Systems™ Mouse IL-13 Culture Media Quantikine ELISA

Mouse IL-13 Culture Media Quantikine ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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Specifications

Specificity	Natural and Recombinant Mouse IL-13
Target Species	Mouse
Conjugate	HRP
Gene ID (Entrez)	16163
Sample Type	Cell Culture Supernatant
Sample Volume	Cell Culture Supernates (50uL)
Synonym	ALRHMGC116789, BHR1interleukin-13, IL13, IL-13 mgC116788, interleukin 13, MGC116786, NC30, P600
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

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Invitrogen™ Human MMP1-Cell Lysate ELISA Kit

ELISA

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Specifications

Protein Family	Cell Structure/Organelle Proteins
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 20 μL
Assay Sensitivity	8 pg/mL
Research Discipline	Extracellular Matrix & Cell Adhesion
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	MMP1
Specificity	The antibody pair provided in this kit recognizes human MMP-1.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Interassay CV	<12%
Gene Symbol	MMP1
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	8-18000 pg/mL
Accession Number	P03956
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	4312
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	CLG, CLGN

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Invitrogen™ Human FGF4 (Cell Lysates) ELISA Kit

ELISA

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Specifications

Protein Family	Growth Factors & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 20 μL
Assay Sensitivity	50 pg/mL
Research Discipline	Apoptosis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	FGF4
Specificity	The antibody pair provided in this kit recognizes human FGF-4.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Interassay CV	<12%
Gene Symbol	Fgf4
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	50-18000 pg/mL
Accession Number	P08620
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	2249
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF

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Invitrogen™ Mouse HGF-Cell Lysates ELISA Kit

ELISA

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Specifications

Protein Family	Chemokines & Receptors
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 50 μL
Assay Sensitivity	400 pg/mL
Research Discipline	Apoptosis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	HGF
Specificity	The antibody pair provided in this kit recognizes mouse HGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	400 pg/mL to 60 ng/mL
Interassay CV	<12%
Gene Symbol	Hgf
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	400-60 ng/mL
Accession Number	Q08048
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	15234
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF

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Invitrogen™ Mouse TIMP1-Cell Lysate ELISA Kit

ELISA

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Specifications

Shipping Condition	Wet or Dry Ice
Protein Family	Cytokines & Receptors
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	TIMP1
Total Assay Time	4 hr 45 min
Assay Sensitivity	3 pg/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	3-1000 pg/mL
Test Time	1 hr 20 min
Accession Number	P12032
Regulatory Status	RUO
Target	TIMP1
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	CLGI; collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EG-1; Embryogenin-1; EPA; EPO; erythroid potentiating activity; erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Metalloproteinase inhibitor; metalloproteinase inhibitor 1; metalloproteinase tissue inhibitor; metalloproteinase tissue inhibitor 1; MMP inhibitor; RP1-230G1.3; Timp; TIMP 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMP-1 protein; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metal proteinases; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases; tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1CLGI; collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EG-1; Embryogenin-1; EPA; EPO; erythroid potentiating activity; erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Metalloproteinase inhibitor; metalloproteinase inhibitor 1; metalloproteinase tissue inhibitor; metalloproteinase tissue inhibitor 1; MMP inhibitor; RP1-230G1.3; Timp; TIMP 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMP-1 protein; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metal proteinases; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases; tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1; Clgi; EPA; TIMP-1; TPA-S1; Timp
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21857
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	Biotin

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Invitrogen™ Mouse VEGF-A-Cell Lysate ELISA Kit

ELISA

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Specifications

Protein Family	Chemokines & Receptors
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 20 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Angiogenesis
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	VEGF
Specificity	The antibody pair provided in this kit recognizes mouse VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	2 pg/mL to 1000 pg/mL
Interassay CV	<12%
Gene Symbol	VEGFA
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	2-1000 pg/mL
Accession Number	Q00731
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	22339
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	Vegf, Vpf

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Invitrogen™ Rat VEGF-A-Cell Lysates ELISA Kit

ELISA

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Specifications

Protein Family	Chemokines & Receptors
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 20 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Oncology
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	VEGF
Specificity	The antibody pair provided in this kit recognizes rat VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Sufficient For	96 Assays
Calibration Range	2 pg/mL to 200 pg/mL
Interassay CV	<12%
Gene Symbol	VEGFA
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	2-200 pg/mL
Accession Number	P16612
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	83785
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	VEGF-A, VEGF164, VPF, Vegf

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Invitrogen™ Human VEGF-A Cell Lysates ELISA Kit

ELISA

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Specifications

Protein Family	Chemokines & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Cell Lysate 50 μL
Assay Sensitivity	10 pg/mL
Research Discipline	Immunology
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	VEGF
Specificity	The antibody pair provided in this kit recognizes human VEGF-A.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	10 pg/mL to 6000 pg/mL
Interassay CV	<12%
Gene Symbol	VEGFA
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	10-6000 pg/mL
Accession Number	P15692
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	7422
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Synonym	RP1-261G23.1, MVCD1, VEGF, VPF

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Invitrogen™ Mouse ShhN (SHH)-Cell Lysates ELISA Kit

ELISA

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Specifications

Shipping Condition	Wet or Dry Ice
Protein Aliases	SHH, HHG-1, Sonic hedgehog protein 19 kDa product, Sonic hedgehog protein 27 kDa product
Protein Family	Other Proteins
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Shh
Total Assay Time	4 hr 45 min
Assay Sensitivity	5 pg/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	4.92-1200 pg/mL
Test Time	1 hr 20 min
Accession Number	Q62226
Regulatory Status	RUO
Target	SHH
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Cell Lysis Buffer,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH; 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	20423
Sample Type	Cell Lysates,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	Biotin

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