HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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MilliporeSigma™ Supelco™ Ascentis™ Express HILIC UHPLC Column, 2 μm Particle Size

Contains a bare silica stationary phase that can be used for traditional normal phase separations or aqueous normal phase chromatography.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	2 μm
Stationary Phase	Silica
Phase	Reversed Phase
Matrix	Silica Phase/Fused-Core Particle Platform
Endcapped	No
Technique	Fused-Core UHPLC
Product Line	Ascentis

MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	2.7 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 9
Technique	Fused-Core HPLC
Product Line	Ascentis

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å Biphenyl HPLC Column, 5 μm Particle Size

Ascentis™ Express 90 Å Biphenyl columns can be used for basic, acidic, or neutral compounds with alternate selectivity from C18 and is especially useful for separating peaks that elute early on C18 and other phenyl phases.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	5 μm
Stationary Phase	Biphenyl (Dimethylbiphenyl) Bonding
Phase	Reversed Phase
Matrix	Biphenyl (Dimethylbiphenyl) Bonding Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 9
Technique	Fused-Core HPLC
Surface Area	90 m2/g
Product Line	Ascentis

MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 5 μm Particle Size

The densely bonded, extensively endcapped dimethyloctyl stationary phase of Ascentis™ Express 5 μm C8 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	5 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 9
Technique	Fused-Core HPLC
Product Line	Ascentis

MilliporeSigma™ Supelco™ Ascentis™ Express RP-Amide HPLC Column, 5 μm Particle Size

Ascentis™ Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis™ Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from 'phase collapse'.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	5 μm
Stationary Phase	Amide, Alkyl
Phase	Reversed Phase
Matrix	Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped	Yes
Technique	Fused-Core HPLC
Product Line	Ascentis