MTT (Thiazolyl Blue Tetrazolium Bromide) is a yellow tetrazolium salt used for measuring cell viability and metabolic activity in laboratory assays. The 5g format is ideal for larger-scale experiments or high-throughput screenings, where MTT is utilized to assess cellular metabolism by measuring the conversion of the tetrazolium salt into purple formazan crystals. This assay is essential in determining cytotoxicity, cell proliferation, and mitochondrial function, making it crucial in pharmacological studies, cancer research, and toxicology testing. The simplicity, low cost, and reliability of the MTT assay make it a widely used method in cell-based research to evaluate the effects of drugs, chemicals, and other treatments on living cells.