3-Indoxyl β-D-Glucoside (IBDG) is a chromogenic substrate used for the detection of β-glucosidase activity in biochemical assays. Upon hydrolysis by β-glucosidase, the substrate produces an indigo-colored product, which is useful for visualizing enzyme activity in bacterial colonies or cell cultures. The 100mg format is ideal for smaller-scale applications and routine laboratory experiments. IBDG is widely used in molecular biology, microbiology, and genetic research to monitor gene expression, screen for β-glucosidase activity, and study enzyme function. This reagent offers a clear, colorimetric result that is easy to interpret, providing researchers with an efficient tool for enzyme assays and gene function analysis.