Color Standards
Apexbio Technology LLC Annexin V-Cy5/SYTOX Green Apoptosis Kit 100assays
Annexin V is a Ca2 dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS resides on the inner surface of the cell membrane s phospholipid bilayer During early stages of apoptosis it translocates from the inner leaflet to the outer surface thus exposing itself to the extracellular environment Cy5-labeled Annexin V can serve as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy within 10-20 minutes SYTOX Green is a non-cell-permeable cyanine fluorescent dye and stains only dead cells It can be used effectively to differentiate normal viable cells from necrotic cells For cells at late apoptotic stages or dead cells SYTOX Green can penetrate the disordered regions of cell membrane reach the cell nucleus embedding into the DNA double helix to form a SYTOX Green-DNA complex resulting in a 500-fold increase of fluorescence intensity with bright green fluorescence
Apexbio Technology LLC Mitochondrial Membrane Potential and Apoptosis Detection Kit 50T
Mito-Tracker Red CMXRos (MitoMark Red I) is a red fluorescent oxidation-sensitive probe that specifically labels mitochondria It can permeate live cell membranes and selectively accumulate in mitochondria When mitochondrial membrane potential decreases the intensity of red fluorescence also diminishes thus enabling evaluation of mitochondrial damage based on fluorescence intensity Mitochondrial Membrane Potential and Apoptosis Detection Kit is designed to simultaneously detect apoptosis and changes in mitochondrial membrane potential by employing Annexin V-FITC (a green-fluorescence apoptosis probe) and Mito-Tracker Red CMXRos Additionally the kit includes Hoechst 33342 which stains cell nuclei Following staining live cells appear positive for red fluorescence and negative for green fluorescence while apoptotic cells appear positive for green fluorescence and exhibit significantly reduced or negative red fluorescence
Apexbio Technology LLC Annexin V-Cy5 Apoptosis Kit 50 assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but during the early stages of apoptosis it is translocated from the inner to the outer leaflet thus becoming exposed on the cell surface Annexin V exhibits high affinity for PS Cy5-labeled Annexin V can be used as a fluorescent probe allowing the detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining process only takes 10 minutes
Apexbio Technology LLC HyperScribe T7 High Yield RNA Synthesis Kit (N1-Methylpseudo-UTP) 100 rxns
Our product the T7 High Yield RNA Synthesis Kit is based on the principle of T7 RNA polymerase and enables efficient in vitro RNA transcription in a relatively short period of time The kit includes the modified nucleotide N1-Methylpseudo-UTP (m1 ) (Catalog B8049) which can replace regular UTP to reduce host cell immune responses and improve RNA stability This kit is designed to produce high-yield RNA transcripts highly specific radiolabeled RNA probes as well as capped RNA dye-labeled or biotin-labeled modified nucleotide RNAs RNAs synthesized by this kit have broad applications in biological experiments such as in vitro translation antisense RNA and RNAi experiments RNA vaccine development RNA structural and functional studies ribozyme biochemistry RNase protein assays and probe-based hybridization blots This kit supports for 25/50/100 reactions at 20 L With 1 g of control template can yield up to 180 g of RNA
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
Apexbio Technology LLC Annexin V-HF488/7-AAD Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes 7-AAD can specifically bind to double-stranded DNA resulting in red fluorescence Normally 7-AAD cannot pass through intact cell membranes however it can penetrate the cell membranes of late-stage apoptotic and dead cells staining their nuclei Combined staining with Annexin V-HF488 and 7-AAD allows differentiation of cells in various stages of apoptosis and dead cells
Apexbio Technology LLC One-step TUNEL Cy5 Apoptosis Detection Kit 100rxns
One-step TUNEL Cy5 Apoptosis Detection Kit is designed for detecting cell nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases are activated cleaving genomic DNA between nucleosomes and resulting in DNA fragmentation into segments approximately 180-200 bp or multiples thereof The fragmented genomic DNA contains exposed 3 -OH ends which are labeled with fluorescent-tagged dUTP in a TdT (Terminal Deoxynucleotidyl Transferase) catalyzed reaction allowing visualization and detection by fluorescence microscopy or flow cytometry (Ex/Em 649 nm/670 nm) This kit has a broad range of applications and can be utilized to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100 X 1ml
Crude cell extracts typically contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins During cell or tissue protein extraction in vitro protein synthesis is significantly inhibited while degradation is enhanced Adding inhibitors against these enzymes can improve yields of intact proteins Protease inhibitor cocktails are used with cell lysates or tissue extracts to enhance protein stability Our protease inhibitor cocktail has broad specificity and serves as a general-use protease inhibitor set suitable for purifying histidine-tagged proteins It contains multiple critical components including AEBSF Aprotinin Bestatin E-64 Leupeptin Phosphoramidon and Pepstatin A with broad inhibition specificity against serine proteases cysteine proteases acidic proteases aminopeptidases and metalloproteases The product is supplied as a ready-to-use solution in DMSO and can be used in WB Co-IP pull-down assays IF IHC and kinase assays
Apexbio Technology LLC Annexin V-FITC/PI Apoptosis Kit 50 assays
Annexin V is a Ca2 -dependent phospholipid-binding protein which specifically binds phosphatidylserine (PS) Under normal conditions PS resides on the inner leaflet of the cell membrane lipid bilayer However during early apoptosis PS flips from the inner to the outer surface of the cell membrane exposing itself to the extracellular environment Annexin V exhibits high affinity for PS and can be labeled with fluorescein isothiocyanate (FITC) FITC-labeled Annexin V serves as a fluorescent probe allowing detection of apoptotic cells by flow cytometry or fluorescence microscopy The staining procedure only takes 10 20 minutes Propidium iodide (PI) binds specifically with double-stranded DNA and emits red fluorescence Normally PI cannot penetrate intact cell membranes However in late apoptotic and dead cells PI can enter through compromised membranes and stain the nuclei When Annexin V-FITC and PI staining are performed simultaneously cells at different stages of apoptos
Apexbio Technology LLC Annexin V-Cy3/SYTOX Green Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but flips from the inner to the outer leaflet during the early stages of apoptosis becoming exposed to the extracellular environment Annexin V has a high affinity for PS By conjugating Annexin V with Cy3 the labeled Annexin V serves as a fluorescent probe enabling detection of apoptosis events via flow cytometry or fluorescence microscopy within only 10 20 minutes staining SYTOX Green is a membrane-impermeable cyanine dye that normally cannot penetrate intact cell membranes However during late-stage apoptosis or necrosis SYTOX Green can cross disrupted regions of the cell membrane reach the nucleus and bind DNA double helix molecules forming SYTOX Green-DNA complexes Binding increases fluorescence intensity over 500-fold generating bright green fluorescence Thus SYTOX Green stains o
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Apexbio Technology LLC Annexin V-Cy3 Apoptosis Kit 50 assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) In normal conditions PS is located on the inner side of the cell membrane lipid bilayer However in the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS and Annexin V labeled with Cy3 can serve as a fluorescent probe for detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 10 minutes
Apexbio Technology LLC Annexin V-Cy5/DAPI Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (Cy5) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence but normally cannot permeate intact cell membranes However DAPI can enter late-stage apoptotic or dead cells and stain their nuclei Simultaneous staining with Annexin V-Cy5 and DAPI allows differentiation of cells at various stages of apoptosis and cell death
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 100x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO
Apexbio Technology LLC Annexin V-HF647/PI Apoptosis Kit 100assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes PI is a dye which specifically binds double-stranded DNA and emits red fluorescence under normal circumstances it cannot penetrate intact cell membranes However in late apoptotic and dead cells PI penetrates the cellular membrane staining the nuclei Dual staining with Annexin V-HF647 and PI enables clear differentiation between early apoptotic late apoptotic and dead cells