Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
TRIS-buffered saline (TBS, 10X), with 1% Triton™ X-100, Thermo Scientific Chemicals
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
HEPES, 0.5M buffer soln., pH 6.5, Thermo Scientific™
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
Tricine-buffered saline (5X), pH 8.0, Thermo Scientific™
CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 20X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
GTE buffer, Thermo Scientific™
For miniprep and DNA purification
TES sodium salt, Thermo Scientific Chemicals
CAS: 70331-82-7 Molecular Formula: C6H14NNaO6S Molecular Weight (g/mol): 251.23 MDL Number: MFCD00065482 InChI Key: GUXMFAHOYNRHBI-UHFFFAOYSA-M Synonym: 2-([Tris(hydroxymethyl)methyl]amino)ethane-1-sulfonic acid sodium salt PubChem CID: 23702152 IUPAC Name: sodium 2-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}ethane-1-sulfonate SMILES: [Na+].OCC(CO)(CO)NCCS([O-])(=O)=O
Transformation buffer-2, pH 7.5, Thermo Scientific™
For transformation of competent cells
Glycylglycine, 0.2M buffer soln., pH 8.0, Thermo Scientific™
CAS: 556-50-3 Molecular Formula: C4H8N2O3 Molecular Weight (g/mol): 132.119 MDL Number: MFCD00008130 InChI Key: YMAWOPBAYDPSLA-UHFFFAOYSA-N PubChem CID: 11163 ChEBI: CHEBI:17201 IUPAC Name: 2-[(2-aminoacetyl)amino]acetic acid SMILES: C(C(=O)NCC(=O)O)N
